نتایج جستجو برای: restriction fragment length polymorphism pcr

تعداد نتایج: 653258  

Journal: :Plankton and Benthos Research 2023

Ghost crabs (genus Ocypode spp.) found on sandy beaches are susceptible with the loss of an ecotone, making them useful as indicators anthropogenic alteration, such seawall construction. On Sendai Bay Pacific coast Tohoku region northern Japan, three species have been sympatrically observed in recent years: O. stimpsoni, ceratophthalmus, and sinensis. However, these only occur from late summer ...

E. Zareian Jahromi, I. Ahrari M. Ghavipisheh M. Ranjbari S. Ahrari S. Khaleghizadeh

Calpastatin (CAST) is a specific inhibiter of Calpains, playing a role in meat tenderization and myogenesis. In the present study the polymorphism of the CAST gene ofKhalkhali goat in Azerbaijan province in Iran was investigated by polymerase chain reaction and restriction fragment length polymorphism technique (PCR-RFLP). Genomic DNA was extracted from whole blood samples collected from 200 Kh...

Journal: :Diabetes 1982
D Owerbach J Nerup

Variant DNA sequences flanking the human insulin gene were found in the Danish population using restriction endonucleases (restriction fragment length polymorphism). The frequencies of these DNA sequences were determined in 47 non-insulin-dependent diabetics and 93 control individuals. We report an association between a restriction fragment length polymorphism of the insulin gene and NIDDM.

2014
Monika Żelazowska Małgorzata Lelonek Wojciech Fendler Tadeusz Pietrucha

INTRODUCTION Numerous hormones, neurotransmitters, and other stimuli exert their biological effect on cellular functioning through heptahelical receptors coupled to G proteins (GPCR - G protein-coupled receptors). Adrenergic receptors that belong to this superfamily of receptors are components of the sympathetic nervous system. They play a pivotal role in blood pressure regulation and myocardia...

2014
Zahra Vali Abbasali Raz Hanieh Bokharaei Mohammad Nabavi Mohammad Hassan Bemanian Mina Sharifi Yazdi Navid Dinparast Djadid

BACKGROUND Asthma is caused by the combination of different factors. Current concepts of asthma pathogenesis emphasize on gene-environment interactions. Mega-genome scanning projects revealed that different Single Nucleotide Polymorphisms (SNPs) are related to asthma susceptibility. rs7216389-T is one of them that is related to childhood asthma and its effect on childhood asthma severity has be...

Journal: :molecular and biochemical diagnosis (journal) 2014
mortaza bonyadi elahe nabat mahnaz sadeghi shabestari mandana rafeey fakhrossadat mortazavi

background: henoch-schönlein purpura (hsp) is an lga mediated small vessel systemic vasculitis disease in children. the etiology and pathogenesis of hsp disease remain unknown. however, environmental and genetic risk factors could play important roles in susceptibility to hsp disease. in this study we investigated the association of 5՛-untranslated region polymorphism (-634g/c) of vegf gene wit...

2014
Nancy Younis Omar Hala Abdel Salam Ali Reem Abdel Hameed Harfoush Engy Hamdy El Khayat

Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A (spa) and coagulase (coa) ...

Journal: :International journal for parasitology 2005
C Sreekumar D E Hill K B Miska M C B Vianna L Yan R L Myers J P Dubey

A Pyrosequencing assay, based on SAG2 gene polymorphisms, was designed for genotyping and detection of multiple infections of Toxoplasma gondii. The assay was tested on samples spiked with DNA from single and multiple genotypes of T. gondii and also on a DNA sample from the brain of a rat with multiple infections. To evaluate the comparative efficacy of the assay, identical samples were also an...

A Al Jawabreh GH KHalili M Tashakori M.H Alimohamadiyan P Jfarpour

Background & Aims: In recent years, molecular methods for characterizing genetic heterogeneity have found a major place in modern approaches. In this study, two different molecular techniques including Restriction Fragment Length Polymorphism (RFLP) and Multi Locus microsatellite typing (MLMT) were carried out in order to evaluate genetic heterogeneity among isolates of Leishmania major in Iran...

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