this study was carried out to investigate effects of copper sulphate (cuso4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and dna damage, after semen dilution and cryopreservation. semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. a total number of 25 samples were used in each...

introduction: in the present investigation, we aimed to study the effect of different concentrations of ascorbate/vitamin c, on membrane integrity, viability, and acrosome reaction of human spermatozoa in presence of ferrous (fe2+) ions as lipid peroxidation (lpo) promoter. material and methods: we used 12 semen samples from healthy donors in shahrekord. the samples were then washed twice with ...

Oxidative stress during freezing-thawing reduces sperm motility, viability, membrane functions and antioxidant capacity. Thymus vulgaris has antioxidant properties due to phenolic compounds. The purpose of current study was to evaluate the effect of Thymus vulgaris extract as a natural antioxidant on post-thawed Moghani ram sperm quality. Semen samples were collected twice a week from four rams...

In this study, two following experiments were performed to improve post-thaw motility and viability of frozen-thawed ram spermatozoa. We examined i) the effects of different concentrations of bovine serum albumin (0, 0.3, 1, 5, 10 and 15% BSA) in semen diluents lacking egg yolk and ii) the effects of four semen diluents, fructose (F: control) and trehalose (T) in semen diluents containing egg y...

four mature stallions were used to study the effect of enzymatic antioxidant catalase on freezability of turkmen stallion's sperm. collected semen from stallions were processed and pooled before being divided treatments (200×106  sperm/ml) and diluted with extenders supplemented with different levels of catalase [0 (control), 100 u/ml (cat100), 150u/ml (cat150) and 200u/ml (cat200)]. exten...

experiment was conducted to evaluate the effect of l-glutamine on coated sperm freezing by using four rams. semen was collected by artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. samples were pooled and centrifuged. after removing supernatant and diluting pellet, samples were split into 5 equal part and added 0, 40, 80, 120 and 160 mm l-glutamine. samples were froze...

Background: Fluoxetine (FLX) is an Antidepressant compound. FLX produces undesired side effects including anxiety, sleep disturbances, sexual dysfunction and gastrointestinal disturbances. The grape is a significant source of nutritional antioxidants, such as polyphenols, anthocyanins, flavonids as well as biologically active dietary components. Present study was performed to investigate the pr...

In vitro supplementation with date seed oil (DSO) can protect spermatozoa against hydrogen peroxide (H2O2)-mediated damage and can improve sperm function, possibly owing to antioxidant properties. We tested the antioxidant effects of DSO on human sperm motility, sperm viability, reacted acrosome and lipid peroxidation assessed in vitro after H2O2-mediated oxidative damage in spermatozoa. Sixtee...

Influences on viability and germination of seeds have been studied intensively for many years, particularly for the numer-ous commercially important agricultural, ornamental, and timber species. High germinating seed lots may differ substantially in field emergence when sown at the same time in the same field, and/or may differ in performance after storage in the same environment or transport t...

the freezing and thawing process not only is associated with serious damage to sperm such as damage to the plasma membrane and the acrosomal membrane but also changes the membrane permeability to some ions including calcium. also, the generation of oxygen free radicals is increased during the freezing-thawing process. the purpose of this study was to evaluate of the effects of trolox as an anti...