As a means of investigating gene function, we developed a robust transcription fusion reporter vector to measure gene expression in bacteria. The vector, pTH1522, was used to construct a random insert library for the Sinorhizobium meliloti genome. pTH1522 replicates in Escherichia coli and can be transferred to, but cannot replicate in, S. meliloti. Homologous recombination of the DNA fragments...

Abstrsct: As a means of investigating gene function, we developed a vigorous transcription fusion reporter vector to measure gene expression in plant. The vector, plasmid, was used to construct a haphazard insert library for the Osmium bsilicum genome. plasmid replicates in Escherichia coli and can be transferred to, but cannot replicate in, S. meliloti. Homologous recombination of the DNA frag...

PURPOSE To develop a system for inducible photoreceptor-specific gene expression in transgenic mice. The tetracycline regulatory system was chosen because it possesses the useful property of direct control of gene expression through use of an exogenous agent, doxycycline, a tetracycline derivative. METHODS Transgenic mice were generated that carried the reverse tetracycline-controlled transac...

Preclinical and clinical tomographic imaging systems increasingly are being utilized for non-invasive imaging of reporter gene products to reveal the distribution of molecular therapeutics within living subjects. Reporter gene and probe combinations can be employed to monitor vectors for gene, viral, and cell-based therapies. There are several reporter systems available; however, those employin...

The filamentous fungus Aspergillus niger is widely employed in the production of citric acid and some industrial enzymes such as phytase, glucoamylase, glucose oxidase. Currently, several genetic modification tools have been developed applied to this fungal species. However, RNA silencing technique less used for A. niger. In present study, an binary vector integrated with Gateway recombination ...

Nucleic acid therapeutics have demonstrated an impressive acceleration in recent years. They work through multiple mechanisms of action, including the downregulation gene expression and modulation RNA splicing. While several drugs based on former mechanism been approved, few target latter, despite promise splicing modulation. To improve our ability to discover novel splicing-modulating therapie...

BACKGROUND Quantifying plant gene expression by flow cytometry (FCM) would allow multidimensional cell-parameter analysis on a per-cell basis, thereby providing insight into the cellular mechanisms of plant gene regulation. Here we sought to establish quantitation by FCM of plant hormone (abscisic acid, ABA)-inducible green fluorescent protein (GFP) expression and to compare the method directly...

Changes in the expression and function of genes drive evolutionary change. Comparing how genes are regulated in different species is therefore becoming an important part of evo-devo studies. A key tool for investigating the regulation of genes is represented by bacterial artificial chromosomes (BAC)-reporter constructs. BACs are large insert libraries, often >100 kb, which thus capture the geno...

Abstract Background Our previous study shows that LINC01278 inhibits the malignant proliferation and invasion of papillary thyroid carcinoma (PTC) cells by regulating miR-376c-3p/DNM3 axis. However, regulation mechanism expression in PTC is still unclear. Methods The luciferase reporter ChIP assays were used to confirm binding LEF-1 putative promoter site gene. RNA immunoprecipitation pulldown ...

UNLABELLED Our goal was to noninvasively measure chemotherapy-induced changes in the expression of critical tumor growth genes. To achieve this goal, we used radionuclide and optical methods to measure changes in human telomerase reverse transcriptase (hTERT) gene expression in tumor cells before and after 5-fluorouracil treatment. METHODS A fusion reporter construct, containing humanized Ren...