The correlation between hip replacement (Hip-Rep) and peripheral arterial disease (PAD) remains uncertain. Thus, we investigated the relationship between Hip-Rep and risk of developing PAD in a nationwide retrospective cohort study.National Health Insurance data were used to assemble a cohort of patients who were diagnosed from 2000 to 2011. Patients with a history of PAD were excluded. A total...

The unwinding reaction catalyzed by the Escherichia coli Rep protein is stimulated by a small 15 kDa protein called Rep helicase stimulatory protein (RHSP)(1). The RHSP-stimulated unwinding reaction catalyzed by Rep protein proceeded at a rapid rate after a time lag of 1-2 min at 37 degrees C. This time lag was eliminated by preincubating RHSP with the DNA substrate, indicating that stimulation...

The pH dependence of the receptor potential (ReP) of isolated layers of photoreceptor cells from the hermit crab compound eye (Eupagurus bernhardus L.) was determined. Measurements were performed, using extracellular electrodes, in the range pH 3.5 —9.5 in three different buffer systems: Tris, glycine, and phosphate. The amplitude of the ReP was highest at pH 7.5 and decreased in more acidic an...

Figure I. a. In the absence of ATP, EFRET distributions of DNA III (Fig. 2) at various [Rep] showed at least four conformations. EFRET was determined from each DNA by averaging the first second of observation. The peak at EFRET=0 is due to inactive acceptor dyes and will not be discussed further. With no Rep in solution, the distribution is peaked at EFRET=0.7, reflecting the DNA-only state (S)...

We study stabilization of finite-dimensional representations the periplectic Lie superalgebras $\mathfrak{p}(n)$ as $n \to \infty$. The paper gives a construction tensor category $Rep(\underline{P})$, possessing nice universal properties among categories over $\mathtt{sVect}$ complex vector superspaces. First, it is abelian envelope Deligne corresponding to superalgebra, in sense arXiv:1511...

Herpes simplex virus type 1 (HSV-1) amplicon vectors are promising gene delivery tools, but their utility in gene therapy has been impeded to some extent by their inability to achieve stable transgene expression. In this study, we examined the possibility of improving transduction stability in cultured human cells via site-specific genomic integration mediated by adeno-associated virus (AAV) Re...

UNLABELLED Adeno-associated virus serotype 2 (AAV2) can efficiently replicate in cells that have been infected with helper viruses, such as adenovirus or herpesvirus. However, in the absence of helper virus infection, AAV2 establishes latency by integrating its genome site specifically into PPP1R12C, a gene located on chromosome 19. This integration target site falls into one of the most gene-d...

The SF1 DNA helicases are multi-domain proteins that can unwind duplex DNA in reactions that are coupled to ATP binding and hydrolysis. Crystal structures of two such helicases, Escherichia coli Rep and Bacillus stearothermophilus PcrA, show that the 2B sub-domain of these proteins can be found in dramatically different orientations (closed versus open) with respect to the remainder of the prot...

Chloronaphthalenes (CNs), due to their structural similarities to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and the other "dioxin-like" compounds, can bind to the aryl hydrocarbon receptor (AhR) and induce a wide range of pleotrophic effects. Relative potency of individual dioxin analogues can be measured relative to that of TCDD. Relative effects potencies (REP) can be based on many responses...

Porcine circovirus (PCV) has been assumed to replicate its genome via the rolling-circle replication (RCR) mechanism because it encodes a Rep protein that contains several amino acid motifs commonly found in other RCR biological systems. Two proteins, Rep and Rep', are essential for PCV DNA replication in mammalian cells. In this work, replicative intermediates of PCV-infected porcine kidney (P...