نتایج جستجو برای: regulatable expression cassette
تعداد نتایج: 878522 فیلتر نتایج به سال:
Introduction: Salmonellosis is an infection caused by eating contaminated food with Salmonella, and it can occur in humans and other animals. Salmonella has acquired the ability to create the infection due to the presence of several virulence genes. One of the virulence genes of salmonella is sipC gene that coding the SipC protein. The aim of this study was creating the gene cassette to genetic...
acute lymphoblastic leukemia (all) is one of the most prevalent hematologic malignancies in children. although the cure rate of all has improved over the past decades, the most important reason for all treatment failure is multidrug resistance (mdr) phenomenon. the current study aims to explain the mechanisms involved in multidrug resistance of childhood all, and introduces atp-binding cassette...
Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...
Pyramiding of <i>cry</i> toxins and methanol producing genes to increase insect resistance in cotton
The idea of enhanced methanol production from cell wall by pectin methyl esterase enzymes (PME) combined with expression cry genes Bacillus thuringiensis as a strategy to improve insect pest control in cotton is presented. We constructed cassette containing two (cry1Fa and Cry32Aa) pme genes, one Arabidopsis thaliana (AtPME), other Aspergillus. niger (AnPME) pCAMBIA1301 plant vector using CAMV-...
Safety and reliability of transgene integration in human genome continue to pose challenges for stem cell-based gene therapy. Here, we report a baculovirus-transcription activator-like effector nuclease system for AAVS1 locus-directed homologous recombination in human induced pluripotent stem cells (iPSCs). This viral system, when optimized in human U87 cells, provided a targeted integration ef...
The tetracycline repressor/operator system was adapted for use in Leishmania donovani. In order to investigate the possible importance and function of open reading frame F (ORFF) in Leishmania donovani strain AG83 that has a single copy of ORFF in the LD1 locus on chromosome 35, we have attempted to generate tetracycline regulatable single allele mutants of ORFF. Leishmania cells expressing the...
A dual promoter probe system based on a tandem bi-cistronic GFP-luxCDABE reporter cassette is described and implemented. This system is assembled in two synthetic, modular, broad-host range plasmids based on pBBR1 and RK2 origins of replication, allowing its utilization in an extensive number of gram-negative bacteria. We analyze the performance of this dual cassette in two hosts, Escherichia c...
BACKGROUND Human adenoviruses are a frequent threat to immunocompromised patients, and disseminated disease is associated with severe morbidity and mortality. Current drugs are not capable of preventing all fatalities, thus indicating the need for alternative treatment strategies. Adenoviruses can be rendered susceptible to antiherpetic prodrugs such as ganciclovir (GCV), upon expression of the...
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