We are interested in the cellular physiological events taking place during shoot regeneration in rice (Oryza sativa L. cv. Tainan 5) callus induced by osmotic stress. At first, the sucrose and starch metabolisms in rice callus were studied because carbohydrates are the main energy source in plant tissue culture. The results showed that fresh weight, water content, cellular water, and osmotic po...

In breeding programs, a large number of F₂ individuals are required to perform the selection process properly, but often few such plants are available. In order to obtain more F₂ seeds, it is necessary to multiply the F₁ plants. We developed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of seeds using 6-benzylaminopurine. To optimize shoot regeneration...

A total of 260 tail blastemata (13 to 15-day regenerates) of adult newts was cultured, with 170 controls, in medium no. 1760 (modified Parker's Medium, CMRL-1415) for 98 h at 20 ±0-5 °C. Various hormones, related to the regeneration process, were added individually and in combinations. If hormones were not included in the culture medium, blastema growth and differentiation did not ensue. The re...

The main purpose of this article is to review role several organic growth additives such Apple juice, coconut water (CW), maize extract, banana homogenate (BH), peptone and protocorms etc which stimulate the multiplication rate various orchid species in vitro multiplication. These supplements help increase number shoots, root leaf culture medium. In many tissue culture, supplements, are most es...

Background: Ferulago angulata is an important medicinal plant and endemic to Iran which is an endangered Plant. Objective: The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat. Metthods: Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was ...

A total of 260 tail blastemata (13 to 15-day regenerates) of adult newts was cultured, with 170 controls, in medium no. 1760 (modified Parker's Medium, CMRL-1415) for 98 h at 20 ±0-5 °C. Various hormones, related to the regeneration process, were added individually and in combinations. If hormones were not included in the culture medium, blastema growth and differentiation did not ensue. The re...

In vitro seedlings were used as explants for protocorm like bodies (PLBs) production which in turn were used for regeneration purpose. PLBs were induced from the base of seedlings (1.0-1.5 cm in size) in MS + BAP (8.88 microM). After 90 days of inoculation, PLBs production rate started declining and most of the PLBs turned into plantlets. Preculture of seedlings in 1.0 microM thidiazuron (TDZ) ...

Objective: To study the intergenus protoplast fusion bioactive marine Streptomyces sp ANS4 with the Escherichia coli for the strain improvement. Methods: Protoplast of both the organisms was prepared by lysozyme treatment and fused using polyethylene glycol (PEG). Fusant colonies were regenerated using regeneration medium where all the regenerated colonies are powdery in consistency. Microscopi...

Axon regeneration in the adult central nervous system (CNS) is limited by several factors including a lack of neurotrophic support. Recent studies have shown that glia from the adult rat CNS, specifically retinal astrocytes and Müller glia, can promote regeneration of retinal ganglion cell axons. In the present study we investigated whether retinal glia also exert a growth promoting effect outs...

Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paro...