The integration of electro and bio-catalysis offers new ways making molecules under very mild, environmentally benign conditions. We show that TEMPO mediated electro-catalytic oxidation alcohols can be adapted to work in aqueous buffers, with minimal organic co-solvent, enabling biocatalytic reductive amination using the AdRedAm enzyme. combined process a approach amine alkylation native alcoho...

The reductive amination of aromatic aldehydes and amines, performed in the Brønsted acidic ionic liquid [Et3NH][HSO4], using sodium borohydride as a reducing agent is reported. In this protocol, plays crucial role efficiently converting to amines excellent yields, without formation side products. presence imine was generated situ from reaction between underwent smooth reduction with borohydride...

NADP-Glutamate dehydrogenase from Aspergillus niger (AnGDH) exhibits sigmoid 2-oxoglutarate saturation. Incubation with 2-hydroxyethyl disulfide (2-HED, the disulfide of 2-mercaptoethanol) resulted in preferential attenuation of AnGDH reductive amination (forward) activity but with a negligible effect on oxidative deamination (reverse) activity, when monitored in the described standard assay. S...

The present study was designed to determine: (i) the role of the reductive amination of alpha-ketoglutarate via the glutamate dehydrogenase reaction in furnishing mitochondrial glutamate and its transamination into aspartate; (ii) the relative incorporation of perfusate 15NH4Cl, [2-15N]glutamine or [5-15N]glutamine into carbamoyl phosphate and aspartate-N and, thereby, [15N]urea isotopomers; an...

Over the past two decades, organocatalytic aldol reaction emerged as a powerful method for enantioselective carbon–carbon bond formation, often offering more efficient, economical and environmentally friendly synthesis of chiral molecules. On other hand, reductive amination is one most important reactions in biologically active compounds drug candidates. Coupling these results combination that ...