OBJECTIVE To explore the radiosensitization effect of overexpression of silent information regulator 6 (SIRT6) on A549 non-small cell lung cancer (NSCLC) cells. METHODS Adenovirus vector Ad-SIRT6 causing overexpression of SIRT6 was established. Western blotting and MTT assay were adopted to detect the level of SIRT6 protein and the inhibitory rate of A549 cell proliferation after different co...

Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relatio...

Effects of laser polarization on fast electron emission are studied from an aluminum target irradiated by ultrashort laser pulses at 2 x 10(16) W/cm(2). Jet emission of outgoing fast electrons collimated in the polarization direction is observed for s-polarized laser irradiation, whereas for p-polarized irradiation highly directional emission of outgoing fast electrons is found in the direction...

Radiotherapy is considered to cause detrimental effects on bone tissue eventually increasing bone loss and fracture risk. However, there is a great controversy on the real effects of irradiation itself on osteoblasts, and the mechanisms by which irradiation affects osteoblast differentiation and mineralization are not completely understood. We explored how X-ray radiation influences differentia...

Human tumour xenografts in a nude rat model have consistently been used as an essential part of preclinical studies for anticancer drugs activity in human. Commonly, these animals receive whole body irradiation to assure immunosuppression. But whole body dose delivery might be inhomogeneous and the resulting incomplete bone marrow depletion may modify tumour behaviour. To improve irradiation-me...

background: the purpose of this work was to investigate the use of polyvinyl chloride (pvc) dyed with methyl red as a high dose radiation dosimetry. materials and methods: commercial pvc films from sabic made from resins pvc57s with thickness of 60 µm and methyl red with a molecular weight of 269.31 were used. irradiation was carried out using a 60co γ-ray generator with sources arranged in rec...

BACKGROUND The aim of the study was to clarify the effect of p53 status of tumor cells on radiosensitivity of solid tumors following accelerated carbon-ion beam irradiation compared with γ-rays or reactor neutron beams, referring to the response of intratumor quiescent (Q) cells. METHODS Human head and neck squamous cell carcinoma cells transfected with mutant TP53 (SAS/mp53) or with neo vect...

The pupae of Sarcophaga peregrina received x-ray in the early pupal stage, and their oxygen consumption was measured throughout the pupal stage. Three patterns of radiation effects were found according to doses. With small irradiation doses, inhibitory effect on oxygen uptake was evident, but the survival time of the pupae was the same as that in non-irradiated, non-fed pupae. With medium irrad...

The aim of this study was to clarify the effects of the muscarinic receptor agonist, cevimeline, on saliva flow and expression of aquaporin5 (AQP5) in submandibular gland after X-ray irradiation. Using a previously established radiation-induced xerostomia model mouse, saliva flow from at 7 days before irradiation to at 28 days after irradiation was investigated in mice that were treated with ce...

Silver nanoparticles were prepared by microwave irradiation of silver nitrate solution with carboxymethyl chitosan as reducing agent and a stabilizer. The optical properties, morphology and structure were characterized using UV–Visible spectrophotometer, transmission electron microscope (TEM) and X-ray diffraction (XRD). Appearance of surface plasmon band at 413 nm indicated the formation of si...