Lysolecithin comprises 9.6 and 21.5% of the phospholipids of the plasma of man and rat, respectively. Ultracentrifugal and gel filtration studies showed that the major portion of the lysolecithin is not found together with the other phospholipids in the plasma lipoproteins. By zone electrophoresis, gel filtration, and ammonium sulfate fractionation, it was found that lysolecithin was consistent...

1. Rat isolated fat-cells were coated with rabbit anti-(rat erythrocyte) antibody and incubated with fresh guinea-pig serum for 25 min at 37 degrees C, which resulted in a more than 95% release of the cytosolic enzyme lactate dehydrogenase. 2. Under these conditions fragmentation of the plasma membrane was examined by following the plasma-membrane markers 5'-nucleotidase, adrenaline-sensitive a...

DEWEY, !hAIAM c. VUScUlUr-eX~rava.scU~ar eXchan,~& of I131 /dasma @tek itz the rat. Am. J. Physiol. 197(2) : 423-43 I. Ig5q.-Rates at which plasma proteins penetrate from the c vascular system into extravascul.ar spaces in tissues was measured with 1131-labeled rabbit gamma globulin, rat gamma and beta globulins, and rat albumin. After vascular mixing was complete, as measured with Cr 51-labele...

Abstract Background Bisphenol A (BPA) causes environmental pollution and is used as a natural antioxidant to protect against chemical side-effects. Costus well-known medicinal plant containing several biologically active compounds. We investigated the protective effects of costus extract toxic BPA in rat testes. Results Biochemical immunohistochemical investigations revealed that bisphenol redu...

Human IL-1, recombinant murine IL-1 and E. coli LPS were found to be potent inducers of plasminogen activator (PA)-inhibitor activity, both in vivo, in rats, as well as in cultured human endothelial cells. In vivo, LPS rapidly and dose-dependently (0.01-1,000 micrograms/kg) increased plasma PA-inhibitor activity. Infusion of IL-1 into rats resulted in a small but significant increase in PA-inhi...

Lysine-epsilon-C(14)-labeled plasma proteins produced by the normal rat and the isolated perfused rat liver have been fractionated by preparative zone electrophoresis. The isolated perfused liver incorporates lysine-epsilon-C(14) into the plasma albumin, alpha globulin, and beta globulin (including fibrinogen) fractions. No significant C(14) incorporation into the trichloracetic acid-precipitab...

Fast and slow rat transferrins were isolated by isoelectric focusing and prepared in their di- and monoferric forms. A comparison of the rates of iron release between fast and slow monoferric transferrins when incubated with reticulocytes or injected in vivo showed no significant difference in the behavior of the two isotransferrin species. Reticulocyte uptake of diferric transferrin resulted i...

A highly sensitive and specific LRH radioimmunoassay is described. Three antisera were compared and characterized. High specificity was shown by 2 of them in that both C- and N-terminals were required for binding when testing LRH analogs. A lower detection limit of about 1 pg could be obtained using a simple organic solvent precipitation method for separation of bound/free antigen. Good correla...

A translational need exists to understand and predict vancomycin-induced kidney toxicity. We describe: (i) a vancomycin high-performance liquid chromatography (HPLC) method for rat plasma and kidney tissue homogenate; (ii) a rat pharmacokinetic (PK) study to demonstrate utility; and (iii) a catheter retention study to enable future preclinical studies. Rat plasma and pup kidney tissue homogenat...

A selective, sensitive and high-throughput liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) method has been developed and validated for the quantitation of Guanfacine in rat plasma. Sample clean-up involved liquid-liquid extraction (LLE) and 100 μL of rat plasma was used. YMC BASIC column (50 mm×2.0 mm, 3.5 µm) was used. Mobile phase used was 10 mM ammonium formate (pH 4.0):acetoni...