Two recent electron microscope studies have demonstrated mitosis in embryonic cardiac fibers . In the chick (3) and the rat (8), both myofibrils and mitotic chromosomes were seen within the same cells. In the normal adult heart, however, cardiac fibers do not usually undergo cell division (2, 7) . The ability of adult cardiac fibers to divide after injury has been a matter of controversy (revie...

BACKGROUND Many methods have been used to assess mitochondrial function. Technetium-99m sestamibi ((99m)Tc-MIBI), a lipophilic cation, is rapidly incorporated into myocardial cells by diffusion and mainly localizes to the mitochondria. The purpose of this study was to investigate whether measurement of (99m)Tc-MIBI signals in animal models could be used as a tool to quantify mitochondrial membr...

BACKGROUND A novel immunomodulator, KRP-203, the molecular structure of which has some similarity to FTY720, has been developed for use in organ transplantation. The present study was designed to investigate the potency and safety of KRP-203 on allograft survival against both acute and chronic rejection in rat skin and heart transplantation. METHODS AND RESULTS KRP-203 significantly prolonged...

The current study examined the contributions of angiotensin-converting enzyme (ACE) vs. chymase to angiotensin II (ANG II) generation in membrane preparations from left ventricles of humans, dogs, rabbits, and rats and from total heart of mice. ACE and chymase activity were measured in membrane preparations extracted with low or high detergent (LD and HD, respectively) concentrations. We hypoth...

Changes in the function of the adrenergic neurons of the heart may be important indicators of the adaptations of an animal to physiologic stress and disease. Rates of loss of norepinephrine (NE) from the heart were considered to be proportional to NE secretion and to adrenergic function. In rat hearts, yohimbine induced almost identical increases in rates of loss of 3H-NE and of 125I-metaiodobe...

Introduction: The present study is aimed to evaluate electrophysiological remodeling of atrioventricular (AV) node and ventricular conduction during experimental atrial fibrillation (AF) model in isolated heart of cirrhotic rats. Methods: Cirrhosis-induced electrophysiological remodeling was evaluated in 24 isolated retrogradely perfused rat hearts in 2 groups (control and cirrhotic). Cirrho...

The low incidence of cardiovascular diseases, including hypertension, in premenopausal women has led to the conclusion that ovarian hormones may have a protective effect on the cardiovascular system. We evaluated the effects of ovariectomy and/or estradiol on sympathovagal balance and heart rate variability (HRV) in female spontaneously hypertensive rats (SHR) with tachycardia and compared them...

To establish a predictive in vitro cardiovascular toxicity and hepatotoxicity screening system, whole cells from the neonatal rat heart and rat hepatocytes were cultured with known cardiotoxic and hepatotoxic compounds and cytotoxicities were assessed. The heart cells were isolated from neonatal SD rats by enzyme digestion and cultured on type I collagen gel. After a several days of preculture,...

Species-specific computer models of the heart are a novel powerful tool in studies life-threatening cardiac arrhythmias. Here, we develop such model aimed at studying infarction injury rat heart, most common experimental system to investigate effects myocardial damage. We updated Gattoni2016 cellular ionic by fitting its parameters data using population modeling approach. Using four selected mo...