A new serologic test for antibodies to Toxoplasma is described, which is based upon inhibition of specific staining with fluorescent antibody. In performing the test, a mixture of the test serum and known fluorescein-labelled antiserum is added to a dried smear of toxoplasms for 1 hour at 37 degrees C. The smear is then rinsed and examined with a fluorescence microscope. Reduction in the bright...

Rich 3D animated explanations can have a powerful impact on students interacting with immersive knowledge-based learning environments. By generating 3D animated explanations in realtime, a learning environment can create engaging explanations that are tailored to individual students. This paper presents the immersive explanation planning framework for generating pedagogically-customized 3D anim...

A cell-based system that detects human enterovirus 71 (HEV71) 3C protease (3C) activity, using fluorescence resonance energy transfer (FRET), was established by creating a stable cell line that constitutively expresses a FRET protein construct consisting of a 3C cleavage motif flanked by a FRET pair of enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP). HEV7...

This paper utilizes data collected using structured questionnaire, focus group discussion, Rapid Participatory Assessment (RPA) and in-depth interview with stakeholders to obtain information from adolescents on the context of their behaviour at family level, work place, school, the society. The aim was to appraise the changes in the socio-cultural context of adolescent sexual and reproductive h...

Conjoint analysis continues to be an area of active research due to its enormous (and often delivered) promise of improved marketing decision-making. However, despite much methodological progress, the literature has remained curiously silent on a fundamental design question: "How does one choose the attributes and levels in the first place?" In this paper, we present a method to support conjoin...

Fluorescent antibody methods were originally introduced by Coons and associates in 1942. Included in this first publication was a procedure for antibody-fluorescein conjugation and a description of fluorescent reactions obtaincd by means of ultraviolet light microscopy. This communication, and later ones by other authors, indicated that fluorescent antibody procedures were practical for rapid a...

Phosphatidylinositol 4,5-bisphosphate (PtdIns[4,5]P 2 ) pools that bind pleckstrin homology (PH) domains were visualized by cellular expression of a phospholipase C (PLC) d PH domain–green fluorescent protein fusion construct and analysis of confocal images in living cells. Plasma membrane localization of the fluorescent probe required the presence of three basic residues within the PLC d PH do...

An accelerated, direct immunofluorescent-antibody procedure was developed for the detection of Salmonella in food products. This method includes pre-enrichment and selective enrichment but eliminates many of the washing and smear treatments present in existing methods. Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, bi...

We investigated extending the use of direct partial hsp65 gene sequencing for the identification of mycobacteria to isolates in primary liquid detection media as an economical, feasible, and more rapid means of identification. During the course of the study, the hsp65 sequence-based identifications for isolates from 670 primary liquid detection media determined to be positive for acid-fast baci...