نتایج جستجو برای: rapd و pcr

تعداد نتایج: 928078  

2017

شور : ،ينيلاب ييامزآراک هعلاطم نيا رد ۲۰ يحارج لمع ديدناك يندومزآ هب يموتكرتسيه دندش هداد ياج لرتنک و شيامزآ هورگ ود رد يفداصت روط . راتفر لوط رد يحارج زا شيپ يتخانش ينامرد ۸ ورگ یارب هسلج شيامزآ ه دوبهب عيرست ظاحل زا هورگ ود ره ،يحارج لمع زا سپ و دش هداد شزومآ رد يرتسب ياهزور دادعت ،يفرصم نكسم يوراد زود نازيم ينعي يمسج تيلاعف هب تشگزاب و ناتسراميب دندش يسررب هرمزور ياه . هداد شهوژپ ياه يرام...

Journal: :Brazilian Journal of Biology 2021

Abstract The principle and the techniques applied in DNA extraction play a pivotal role obtention of purified genetic material. present study investigates efficiency eight protocols Hypostomus commersoni, an essential component South American freshwater ichthyofauna. quality samples was assessed through spectrophotometry, gel electrophoresis, PCR-RAPD markers amplification. influenced both by m...

2005
H. T. L. R L.

Research to determine patterns of genetic differentiation among and within field populations of Crom.um quercuum f. sp.firsifome using RAPD markers is currently underway in the molecular genetics laboratory at the Southern Institute of Forest Genetics. Fungal tissue was collected as a drop of spermatia or scrapings of a discrete hymenium fiom a single gall on each tree sampled at a location. Co...

1998
C. Weng T. L. Kubisiak M. Stine

Sequence characterized amplifled region (SCAR) markers were derived from random amplified polymorphic DNAs (RAPDs) that segregate in a longleaf pine x slash pine Fi family. Nine RAPD fragments, five from longleafpine and four from slash pine, were cloned and end sequenced. A total of 13 SCAR primer pairs, with lengths between 17 and 24 nucleotides, were developed. Nine (for SCAR loci FGPO04, FG...

2012
Balakrishnan Vasanthakumari Premkrishnan Vadivel Arunachalam

RAPD is a simple dominant marker system widely used in biology. Effectiveness of RAPD can be improved by selecting and redesigning primers whose priming sites occur in target sequence(s) of gene or organism at optimum distance. We developed software that uses sequences of random decamer primers and nucleotide sequence(s) as two input files. It locates the priming sites in input sequences and ge...

ژورنال: دنیای میکروب ها 2014

سابقه و هدف: امروزه مایکوباکتریوم های غیرتوبرکلوزی (NTM)، به دلیل افزایش شیوع بیماری زایی و ظهور مقاومت های آنتی بیوتیکی اهمیت فراوان یافته اند. رایج ترین مایکوباکتریوم غیرسلی در ایران ، مایکوباکتریوم فورچوئیتوم می باشد. این مطالعه با هدف شناسایی مولکولی و بررسی تنوع ژنتیکی در میان جدایه های بالینی مایکوباکتریوم فورچوئیتوم در ایران با استفاده از روش RAPD-PCR انجام شد. مواد و روش ها: این مطالعه...

Journal: : 2023

RAPD-PCR method was used for systematic study and revealing the genetic relationships in Malus by using 7 apple cultivars from some mid Iraqi regions, DNA of fresh leaves extracted modified protocol CTAB, 22 prechosen random decamer primers were applied to detect genotypes. four gave reproducible appeared polymorphism RAPD profile, a total 48 bands produced out which 36 polymorphic, results ari...

2018

کچ ی هد پ ی ش مز ی هن ه و فد : ساسا د مردنس رد نامرد ي سفنت سرتس ي ظنت نادازون داح ي سکا لدابت م ي و نژ د ي سکا ي د هدوب نبرک تسا طسوت هک کبس اـه ي ناـمرد ي فلتخم ي هلمجزا لکتورپ INSURE ماجنا م ي دوش ا اذل . ي هعلاطم ن فدهاب اقم ي هس عضو ي ت اه ي ندب ي عضو رب رمد و زاب قاط ي سفنت ت ي هـب لاتـبم سراـن نادازون ردنس د م ي سفنت سرتس ي لکتورپ اب نامرد تحت داح INSURE ماجنا درگ ...

BACKGROUND: Staphylococcus aureus is a highly versatile pathogen of a large number of domestic ani- mals, including avian species. There is limited information about S. aureus isolated from companion and wild birds in Iran. OBJECTIVES: The aim of this study was to determine drug resistance and random-amplified polymorphic DNA-PCR (RAPD-P...

2009
A Tazumi Y Maeda T Buckley BC Millar CE Goldsmith JSG Dooley JS Elborn M Matsuda JE Moore

Clinical isolates (n = 63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n = 24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genoty...

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