Raman Spectroscopy was evaluated as a non-invasive method of analysis of sperm DNA and the influence of UV irradiation on the sperm. The results show that Raman Spectroscopy, combined with multivariate analysis provide the reproducible and accurate information on DNA of sperm and the effect and location of damage.

In this research, tellurium (Te) film with thicknesses of 100-250 nm were deposited on ceramic substrates by thermal evaporation at 373 K. The thickness of the film was determined by Rutherford backscattering spectroscopy. The influence of the thickness on the structural, morphological and molecular bonds was characterized using XRD, scanning electron microscope, and Raman spectroscopy. The XRD...

The advent of robust, rugged, and current Good Manufacturing Practices (cGMP) compliant hand-held Raman spectrometers provides a wealth of opportunities for the analytical pharmaceutical chemist to bring the laboratory to the sample. This paper discusses the use of hand-held Raman spectrometers for the development of qualitative chemical identification methods for a number of well-known pharmac...

[Abstract] Single-laser-shot temperature measurements at a data rate of 1 kHz are demonstrated using femtosecond coherent anti-Stokes Raman scattering (CARS) spectroscopy. The excitation of gas-phase Raman lines with spectral widths of 3 GHz by pump and Stokes beams with spectral widths of 3000 GHz is very efficient provided that the pump and Stokes beams are Fouriertransform-limited. The singl...

Interest in the surface-enhanced Raman scattering (SERS) of 4-aminobenzenethiol (4-ABT) has surged recently. The SERS spectral features are highly dependent on the measurement conditions; a notable example is the appearance of b2-type bands that are not evident in the normal Raman (NR) spectrum. In an effort to discover new information and make any necessary corrections, we measured the Raman s...

The Raman spectra of aqueous ammonia solutions have been obtained between -40 and 25 degrees C. The Raman spectrum of neat water was also obtained at 25 degrees C and is characterized by two broad peaks observed at 3200 and 3400 cm(-1). The spectrum due to water is subtracted to determine the NH(3) spectrum at all temperatures. In ammonia-water solutions, the spectrum shows three features at me...

Cellular differentiation proceeds along complicated pathways, even when it is induced by extracellular signaling molecules. One of the major reasons for this complexity is the highly multidimensional internal dynamics of cells, which sometimes causes apparently stochastic responses in individual cells to extracellular stimuli. Therefore, to understand cell differentiation, it is necessary to mo...

Establishing a standard Raman spectral database for minerals and related inorganic crystals is a very important basis for further increasing the applications of Raman spectroscopy in the geosciences. However, the Raman spectral pattern of a crystal is a function not only of its composition and structure but also of the scattering geometry during the measurement. Therefore, the standard Raman sp...