Background: Short time preservation of semen is common in animals (e.g. horse or pig etc…) which their sperm in known as freezing sensitive sperm. It also may use for short transportation of other species to be cryopreserved or use in AI or IVF programs. The aim of the present study was to evaluate effects of duration of cool storage on goat epididymal sperm. Materials and Methods: Total number...

OBJECTIVE To evaluate the effect of different fractions of Black Maca (Lepidium meyenii), obtained from the hydroalcoholic extract, on spermatogenesis. DESIGN Animal study. SETTING Animal and laboratory facilities at a university. ANIMAL(S) Forty two adult male rats from the Holtzman strain (3 months old). INTERVENTION(S) Hydroalcoholic extract of Black Maca was partitioned with the fol...

The purpose of the present study was to determine the effect of a single oral dose of carbendazim (CARB) on the frequencies of numerical chromosome aberrations in sperm and on micronuclei in peripheral blood erythrocytes of rats. Dual colour FISH on epididymal sperm of rats treated 31 days before sacrifice (0, 50, 150, 450 and 800 mg/kg body wt CARB in corn oil), corresponding to exposure durin...

Several decades ago it was reported that when adult male rats were exposed to a single injection of 50 mg/kg body weight ethane dimethanesulfonate (EDS) and mated with untreated females, average litter size was significantly reduced as early as 2 weeks later. Recently, we demonstrated that EDS exerts multiple effects in the epididymis of adult rats. Some of these effects were independent of red...

The basis for the unusual properties of the catalytic subunit (C) of ram sperm cAMP-dependent protein kinase was investigated. Ram sperm C was purified and found by mass spectrometry (MS) to be approximately 890 Da smaller than Calpha, the predominant somatic isoform. Partial internal amino acid sequence from ram sperm C was an exact match to that of bovine Calpha, but differed from the predict...

Introduction: We aimed to determine pregnancy and miscarriage rates following intracytoplasmic sperm injection (ICSI) cycles using retrieved epididymal and testicular sperm in azoospermic men and ejaculated sperm in oligospermic and normospermic men. Materials and Methods: This retrospective study was carried out on 517 couples who underwent ICSI. They included 96 couples with azoospermia and 4...

ESP1/SPESP1 is a testis-specific, postmeiotic gene expressed in round spermatids that encodes equatorial segment protein 1, an intra-acrosomal protein found in the acrosomal matrix and on the luminal surface of the inner and outer acrosomal membranes within the equatorial segment domain of mature spermatozoa. A comparison of testicular protein extracts with caput, corpus, and caudal epididymal ...

Problem statement: To identify the metabolic reaction-glycolysis or oxidative phosphorylation that is mainly involved in the production of energy required for rat sperm mobilization. Approach: Epididymal sperm were collected from Wistar rats and extended in lactatecontaining or lactate-free raffinose-modified Krebs-Ringer Bicarbonate solution (mKRB)-egg yolk medium supplemented with 0, 1, 2, or...

Sperm motility stimulation is a pharmacological treatment used to improve sperm motility and treatment outcome in frozen-thawed epididymal and testicular spermatozoa. Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular adenosine triphosphate (ATP; molecule that ...