Intrinsically disordered regions (IDRs) play important roles in proteins that regulate gene expression. A prominent example is the intracellular domain of the Notch receptor (NICD), which regulates the transcription of Notch-responsive genes. The NICD sequence includes an intrinsically disordered RAM region and a conserved ankyrin (ANK) domain. The 111-residue RAM region mediates bivalent inter...

The root apical meristem (RAM) determines both RAM activity and the growth of roots. Plant roots are constantly exposed to adverse environmental stresses that can cause DNA damage or cell cycle arrest in the RAM; however, the mechanism linking root meristematic activity and RAM size to the DNA damage response (DDR) is unclear. Here, we demonstrate that a loss of function in RCC1/UVR8/GEF-Like 3...

Sixteen ram lambs were fed 0, 50 or 200 ppm supplemental vanadium daily as NH4VO3 for 90 d and coccygeal vertebrae were sampled at 0, 15, 30, 6 0 a n d 90 d after the imposition of dietary treatments. Vertebral vanadium content was elevated (P<.05) after exposure to 200 ppm added vanadium in the diet for 15 d, but nonsignificant increases occurred thereafter. Increasing dietary vanadium increas...

Milena de Sousa Nascimento, Viviane Gianluppi Ferro & Ricardo Ferreira Monteiro Universidade Federal do Rio de Janeiro, Instituto de Biologia, Departamento de Ecologia, Laboratório de Ecologia de Insetos, CP 68020, 21941-590, Rio de Janeiro, RJ, Brazil. Universidade Federal de Goiás, Instituto de Ciências Biológicas, Departamento de Ecologia, 74690-900, Goiânia, GO, Brazil. Corresponding author...

BACKGROUND Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting biomass growth and recombinant-protein production. With the aim to improve mixing and aeration in shake flask cultures, we analyzed cultures subjected to OM and the novel resonant...