Objective(s): Malignant melanoma is the most lethal type of skin cancers with unfavorable prognosis. Alpha-MSH peptide analogues have a high affinity for melanocortine-1 (MC1) receptors on melanocytes overexpressing in malignant melanoma cells. Pre-clinical studies have shown promising results for radiolabeled MSH imaging in this malignancy. The purpose of this study is to assess the diagnostic...

Breast cancer is one of the most frequent and aggressive primary tumors among women of all races. Matrix metalloproteinase (MMPs), a family of zinc- and calcium-dependent secreted or membrane anchored endopeptidases, is overexpressed in varieties of diseases including breast cancer. Therefore, noninvasive visualization and quantification of MMP in vivo are of great interest in basic research an...

Membrane type-1 matrix metalloproteinase (MT1-MMP or MMP-14) plays an important role in adverse cardiac remodelling. Here, we aimed to develop radiolabeled activatable cell penetrating peptides (ACPP) sensitive to MT1-MMP for the detection of elevated MT1-MMP levels in adverse cardiac remodelling. Three ACPP analogs were synthesized and the most potent ACPP analog was selected using MT1-MMP sen...

Targeting neuroendocrine tumors expressing somatostatin receptor subtypes (sst) with radiolabeled somatostatin agonists is an established diagnostic and therapeutic approach in oncology. While agonists readily internalize into tumor cells, permitting accumulation of radioactivity, radiolabeled antagonists do not, and they have not been considered for tumor targeting. The macrocyclic chelator 1,...

introduction: the gastrin releasing peptide (grp) receptor is over expressed in a variety of common human tumors. radiolabeled bombesin analogues have exhibited high binding affinity for these receptors. the aim of this study was to assess the value of a new 99mtc-bombesin analog in the differentiation of malignant from benign breast tumors. methods: 99mtc-bombesin scans were performed in 21 pa...

An Arabidopsis peptide transport gene was cloned from an Arabidopsis cDNA library by functionally complementing a yeast peptide transport mutant. The Arabidopsis plant peptide transporter (AtPTR2) allowed growth of yeast cells on dipeptides and tripeptides but not peptides four residues and higher. The plant peptide transporter also conferred sensitivity to a number of ethionine-containing, tox...

The reagent 1-ethyl-3-(3-[14C]trimethylaminopropyl)carbodiimide (ETC) was used to identify specific carboxyl groups on the cytochrome bc1 complex (ubiquinol-cytochrome c reductase, EC 1.10.2.2) involved in binding cytochrome c. Treatment of the cytochrome bc1 complex with 2 mM ETC led to inhibition of the electron transfer activity with cytochrome c. Sodium dodecyl sulfate-polyacrylamide gel el...

  Introduction: Cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. Rapid diagnosis of the thrombosis can be an essential step in management of the stroke. Methods: In this work a recently developed radiolabeled streptokinase (STP) tracer was evaluated in an animal thrombotic model using SPECT imaging and biodis...

pr81 is a monoclonal antibody that binds with high affinity to muc1 that over expressed on breast tumors. pr81 is considered a suitable targeting molecule that was radiolabeled using cu-64 for positron imaging studies. the monoclonal antibody was conjugated with dota moiety and after purification was evaluated for radiochemical purity, immunoreactivity, cell toxicity and structure integrity as ...