introduction: muslims fast from dawn to dusk during ramadan. the effects of prolonged food deprivation on endocrine hormones have been studied in healthy adults but no previous study has investigated this effect on children. this study aimed to evaluate the feasible changes in serum level of thyroxin (t3), tetraiodothyronin (t4), thyroid stimulating hormone (tsh) and body composition in pre-men...

We expect and encourage narrow performance ranges within-laboratory, since it is here that the greatest control over laboratory error is possible. This is well illustrated by Gilbert (2), where he indicates that about two-thirds of the inter-laboratory error is attributable to within-laboratory performance characteristics. We thank Dr. Bogdan for his letter and are grateful for the opportunity ...

We have evaluated four current Food and Drug Administration-cleared rapid tests for human immunodeficiency virus (HIV)-specific antibodies with a panel of specimens from recently infected individuals. Recent infection was detected by RNA-based screening coupled with enzyme immunoassay-based testing. We found that the sensitivities of the various rapid tests vary greatly with regard to their abi...

OBJECTIVE The aim of this study was to evaluate the association between lipids and insulin concentration in adolescents. MATERIAL AND METHODS A cross-sectional study of 350 adolescents aged 14-19 years old from a public high school in Guadalajara, in the state of Jalisco, Mexico, was conducted. Fasting insulin concentration was determined using microparticle enzyme immunoassay; total choleste...

Morpho-syntactic patterns device encompasses a subcategory of the cohesive devices that assists hearers to have an adequate mental representation for understanding speech. This article investigates the morpho-syntactic patterns employed in some Persian live sport radio and TV programs adapting Dooley and Levinsohn’s theoretical and analytical framework. The research data includes around 30,000 ...

A solid-phase immunoassay for terminal deoxynucleotidyl transferase has been developed using a primary antibody-coated polystyrene bead and secondary antibody conjugated with horseradish peroxidase. The immunoassay was compared with assays for enzyme activity and detection of antigen with immunofluorescence using cells from peripheral blood and bone marrow from patients with leukemia or lymphom...

We describe the Ames Fluorescent Enzyme Immunoassay procedure for the detection of phenytoin in serum, and compare the results with it with those by enzyme immunoassay(EMIT), "high-pressure" liquid chromatography, and the Dupont aca. Sera from 48 patients who were receiving phenytoin were promptly analyzed in duplicate by EMIT, frozen, then assayed within four weeks by the other three methods. ...

We measured the concentrations of phenobarbital, phenytoin, primidone, ethosuximide, antipyrine, and caffeine in paired samples of saliva and plasma by gas chromatograph-mass spectrometer-computer (GC/MS/COM) and enzyme immunoassay. Mixed saliva was collected for the antipyrine and caffeine studies, parotid saliva for the phenobarbital, primidone, ethosuximide and phenytoin studies. The saliva/...

A homogeneous substrate-labeled fluorescent immunoassay has been applied to the measurement of phenytoin concentrations in human serum. We coupled a fluorogenic enzyme substrate, galactosyl-umbelliferone, covalently to a derivative of phenytoin. Under assay conditions, this drug-substrate conjugate was nonfluorescent but became fluorescent upon hydrolysis catalyzed by bacterial beta-galactosida...