In the present work, the inhibitory effect of carbon monoxide (CO), generated by tricarbonyldichlororuthenium (II) dimer [CO-releasing molecule (CORM-2)], on the toxicity of radiation-induced bystander effect (RIBE) after alpha-particle irradiation was studied in a mixed coculture system. CO (CORM-2) treatment showed a significant inhibitory effect to the formation of p53 binding protein 1 (BP1...

In this study, Chinese hamster ovary (CHO) cells were cultured in custom-made petri dishes with thin PADC films as substrates. Alpha particles with energies of 5 MeV were then irradiated from the bottom of PADC films. The DNA strand breaks in the bystander cells induced by irradiation were quantified with the use of terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay. To study th...

Radiotherapy has a significant impact on the local tumor environment and its distant component. Abscopal effect is the bystander effect of radiotherapy observed at a site distant to that irradiated within the same subject. Abscopal effect even though described, is not a common clinical event. We report a documented observation of abscopal effect in a patient of Chronic Lymphocytic Leukemia duri...

Radiation-induced bystander signaling has been found to occur in live rainbow trout fish (Oncorhynchus mykiss). This article reports identification of key proteomic changes in a bystander reporter cell line (HaCaT) grown in low-dose irradiated tissue-conditioned media (ITCM) from rainbow trout fish. In vitro explant cultures were generated from the skin of fish previously exposed to low doses (...

This study investigated the role of DNA double strand breaks and DNA base damage in radiation-induced bystander responses in Chinese hamster ovary (CHO) cell lines. Two CHO repair-deficient clones, xrs5 (DNA double strand break repair-deficient) and EM9 (DNA base excision repair-deficient) were used in addition to the wild type (CHO). The Gray Cancer Institute ultrasoft X-ray microprobe is a po...

The concept of radiation-induced hormesis, whereby a low dose is beneficial and high detrimental, has been gaining attention in the fields molecular biology, environmental toxicology radiation biology. There growing body literature that recognises importance hormetic response not only field, but also with agents. However, there continuing debate on magnitude mechanism response, which could make...

The effects of radiation on human tissues are not just local as once believed but they can appear even outside of the irradiated field [1-5]. Based on what has been published, both from in vitro and in vivo studies [6,7], it is known that those effects are at least regional. The irradiated tumor tissue may communicate, in some way, radiation effects to the not irradiated neighboring or distant ...

OBJECTIVE The objective of our study was to explore a possible molecular mechanism by which ultraviolet (UV) biophotons could elicit bystander responses in reporter cells and resolve the problem of seemingly mutually exclusive mechanisms of a physical UV signal & a soluble factor-mediated bystander signal. METHODS The human colon carcinoma cell line, HCT116 p53 +/+, was directly irradiated wi...