We constructed a fully automated assay for the cobalamin-dependent enzyme methylmalonyl coenzyme A (CoA) mutase. The assay involves preincubation of the enzyme with adenosylcobalamin, incubation with substrate, termination of the reaction by adding trichloroacetic acid, filtration to remove precipitated protein, and finally analysis of the filtrate (containing methylmalonyl CoA and the product ...

The National Science Foundations Global Environment for Network Innovations (GENI) is an effort to build an environment for large-scale networking experimental research in novel network services and architectures. Deploying an environment to support these research activities requires a platform of sliceable, reservable compute and network resources that are deeply programmable, widely deployed,...

INTRODUCTION Active school transport, such as by walking or biking, increases physical activity levels, which has health and academic benefits for children. We examined school demographic and other characteristics to determine their association with the percentage of students who walk or bike to school. METHODS We analyzed data from the Centers for Disease Control and Prevention's 2014 School...

For frequent disk I/O and large data transmissions among different racks and physical nodes, intermediate data communication has become the most important performance bottle-neck in most running Hadoop systems. This paper proposes a reduce placement algorithm called CORP to schedule related map and reduce tasks on the near nodes of clusters or racks for data locality. Because the number of keys...