Close similarity between the rat native low-conductance K(+) channel in the apical membrane of renal cortical collecting duct principal cells and the cloned rat ROMK channel strongly suggest that the two are identical. Prominent features of ROMK regulation are a steep pH dependence and activation by protein kinase A (PKA)-dependent phosphorylation. In this study, we investigated the pH dependen...

We construct an upper bound for the following family of functionals {Eε}ε>0, which arises in the study of micromagnetics: Eε(u) = ∫ ε|∇u|2 + 1 ε ∫ R2 |Hu |2. Here is a bounded domain in R2, u ∈ H1( , S1) (corresponding to the magnetization) and Hu , the demagnetizing field created by u, is given by { div (ũ + Hu) = 0 in R2 , curl Hu = 0 in R2 , where ũ is the extension of u by 0 in R2 \ . Our u...

R1 and R2 are non-long-terminal repeat retrotransposable elements that insert into specific sequences of insect 28S ribosomal RNA genes. These elements have been extensively described in Drosophila melanogaster. To determine whether these elements have been horizontally or vertically transmitted, we characterized R1 and R2 elements from the seven other members of the melanogaster species subgro...

For simplicity, we adopt the following convention: x, x0, y, y0, r are real numbers, z, z0 are elements of R2, Z is a subset of R2, f , f1, f2 are partial functions from R2 to R, R is a rest, and L is a linear function. Next we state two propositions: (1) domproj(1, 2) = R2 and rng proj(1, 2) = R and for all elements x, y of R holds (proj(1, 2))(〈x, y〉) = x. (2) domproj(2, 2) = R2 and rng proj(...

Some approaches to the synthesis of tetracycline are described. Tetracycline [(I), R' = Me, R2 = R3 = H] is a member of the important group of tetracycline antibiotics. Other members of this group of medicinal importance are aureomycin [(I). R' = Me, R2 = H, R3 = Cl], terramycin [(I), R' = Me, R2 = OH, R3 = H] and 6-demethyltetracycline [(I)], R1 = R2 = R3 = H]. All these compounds represent ch...

(4.14 days to 1.49 days, t = 0.95; P = .37) or clinic capacity, de finedas thesumofappointment slots allocated toagivenclinic or location (1612.6 to 1722.8, t = –1.139;P = .29).Therewasa significant correlation between the quantity of SFT consultationscompletedand thepercentageofnewpatientsbeing seen at theMDCwithin30days (R2 = 0.88;P < .05),newpatientwait times (R2 = 0.95; P < .001), and perce...

PURPOSE To study changes of iron content in basal ganglia in Parkinson's disease (PD) through a three-year longitudinal follow-up of the effective transverse relaxation rate R2*, a validated MRI marker of brain iron content which can be rapidly measured under clinical conditions. METHODS Twenty-seven PD patients and 26 controls were investigated by a first MRI (t0). Longitudinal analysis was ...

TRAIL is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells. Based on homology searching of a private database, a receptor for TRAIL (DR4 or TRAIL-R1) was recently identified. Here we report the identification of a distinct receptor for TRAIL, TRAIL-R2, by ligand-based affinity purification and subsequent molecular cloning. TRAIL-R2 ...

Non-LTR retrotransposons insert into eukaryotic genomes by target-primed reverse transcription (TPRT), a process in which cleaved DNA targets are used to prime reverse transcription of the element's RNA transcript. Many of the steps in the integration pathway of these elements can be characterized in vitro for the R2 element because of the rigid sequence specificity of R2 for both its DNA targe...