نتایج جستجو برای: quantitative rt pcr

تعداد نتایج: 481030  

Journal: :Applied and environmental microbiology 2003
Gwy-Am Shin Mark D Sobsey

Norwalk virus and other human caliciviruses (noroviruses) are major agents of gastroenteritis, and water is a major route of their transmission. In an effort to control Norwalk virus in drinking water, Norwalk virus reduction by bench-scale ozone disinfection was determined using quantitative reverse transcription (RT)-PCR for virus assays. Two other enteric viruses, poliovirus 1 and coliphage ...

2012
Michael J. Moser Robert A. DiFrancesco Krishne Gowda Audrey J. Klingele Darby R. Sugar Stacy Stocki David A. Mead Thomas W. Schoenfeld

Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. Deep sequencing and functional screening of viral metagenomic DNA from a near-boiling thermal pool identified clones expressing thermostable DNA polymerase (Pol) activity. Among these, 3173 Pol demonstrated both high thermostability and innate reverse transcriptase (RT) activity. We describe the b...

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