Pyrimidine derivatives are associated with broad spectrum of biological activities including anti-microbial, anticancer, anti-inflammatory etc. This prompted us to use compounds containing pyrimidine moiety and to evaluate for anti microbial activity. The present study is an in silico approach that aims at finding out the potency of pyrimidine derivatives against multidrug resistant protein of ...

Pyrimidine nucleotides are of general importance for many aspects of cell function, but their role in the regulation of biosynthetic processes is still unclear. In this study, we investigate the influence of a decreased expression of UMP synthase (UMPS), a key enzyme in the pathway of de novo pyrimidine synthesis, on biosynthetic processes in growing potato (Solanum tuberosum) tubers. Transgeni...

The antibacterial agent, trimethoprim, is normally used synergistically with sulfonamides. Its use is associated with idiosyncratic reactions including liver toxicity and agranulocytosis. In this study, we demonstrated that trimethoprim was oxidized by activated human neutrophils, as well as a combination of myeloperoxidase/hydrogen peroxide/chloride or hypochlorous acid, to a reactive pyrimidi...

Some of the factors responsible for the control of pyrimidine nucleotide biosynthesis in intact cells have been investigated using rat hepatoma cells grown in continuous culture. The addition of uridine (0.5 mM) to culture medium causes a decrease of approximately 70% in the rate of de nouo pyrimidine nucleotide biosynthesis, as determined by incorporation of WO 2. Maximum inhibition of W02 inc...

A series of investigations using mutants of the fungus Neurospora has provided ample evidence that the pyrimidine orotic acid has a significant role in the biological synthesis of the ribose nucleosides uridine and cyti-dine.'-4 By use of isotopically labeled orotic acid several investigators5-8 have recently demonstrated that a similar conclusion may be drawn concerning a function of this pyri...

Reaction of copper(II) thiocyanate with pyrimidine leads to the formation of the new ligand-rich 1:2 (1:2 = ratio metal salt to ligand) copper(II) compound [Cu(NCS)(2)(pyrimidine)(2)](n) (1). Its crystal structure was determined by X-ray single crystal investigations. It consists of linear polymeric chains, in which the Cu(2+) cations are mu-1,3 bridged by the thiocyanato anions. The pyrimidine...

Canine cyclic hematopoiesis is an autosomal recessive disease characterized by regular 11-13-d cycles of the neutrophil, reticulocyte, and platelet counts caused by a defect in regulation of marrow stem cell proliferation. Treatment with lithium abrogates cycling of the cell counts in these grey collie dogs. Aware of the defective lymphopoiesis associated with adenosine deaminase and purine nuc...

We have shown previously that Li-Fraumeni syndrome fibroblasts homozygous for p53 mutations are deficient in the removal of UV-induced cyclobutane pyrimidine dimers from genomic DNA, but still proficient in the transcription-coupled repair pathway (Ford, J. M., and Hanawalt, P. C. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 8876-8880). We have now utilized monoclonal antibodies specific for cycl...

In the adult nervous system, the major source of nucleotide synthesis is the salvage pathway. Uridine is the major form of pyrimidine nucleosides taken up by the brain. Uridine is phosphorylated to nucleotides, which are used for DNA and RNA synthesis as well as for the synthesis of membrane constituents and glycosylation. Uridine nucleotides and UDP-sugars may be released from neuronal and gli...

Some of the factors responsible for the control of pyrimidine nucleotide biosynthesis in intact cells have been investigated using rat hepatoma cells grown in continuous culture. The addition of uridine (0.5 mM) to culture medium causes a decrease of approximately 70% in the rate of de nouo pyrimidine nucleotide biosynthesis, as determined by incorporation of WO 2. Maximum inhibition of W02 inc...