A selective and simultaneous staining method for nuclear apparatus and cytoplasmic membrane of some bacteria has been presented. Nuclear apparatus is stained with basic fuchsin after hydrolysis with I N HCl and cytoplasmic membrane is restained with Victoria blue 4R after treating with saturated mercuric chloride. By this method, the nuclear apparatuses of B. subtilis, Sal. typhi 57 and Staph. ...

The present work uses a scanning electrochemical microscopy technique to study systems containing the membrane-bound reaction center protein (RC) from the purple photosynthetic bacteria Rhodobacter spheroides to chromatophores (spherical reorganization of cell membrane following its mechanical rupture) and liposomes (reconstituted membrane systems at lower degree of complexity). Scanning electr...

Chemical modification and electron spin resonance techniques were used to study the topography of carboxyl residues in purple membranes. The results showed that buried carboxyl groups are )ocated in hydrophobic protein domains at least 16 A from the membrane surface, and that the carboxyl-terminal tail is partially immobilized. Carboxyl groups on bacteriorhodopsin in purple membranes...

The absorption spectra of visual purple solutions extracted by various means were measured with a sensitive photoelectric spectrophotometer and compared with the classical visual purple absorption spectrum. Hardening the retinas in alum before extraction yielded visual purple solutions of much higher light transmission in the blue and violet, probably because of the removal of light-dispersing ...

Structural models of membrane proteins can be refined with sets of multiple orientation constraints derived from structural NMR studies of specifically labeled amino acids. The magic angle oriented sample spinning (MAOSS) NMR approach was used to determine a set of orientational constraints in bacteriorhodopsin (bR) in the purple membrane (PM). This method combines the benefits of magic angle s...

A 70 year old lady of recurrent ischaemic stroke on a chronic urinary catheter presented with purple discoloration of her catheter bag. Clinically, she was febrile. The urinary catheter drainage bag (Panel A) was purple stained. Her urine pH was alkaline at 7.5 with leucocytes present. The urine culture grew Klebsiella pneumoniae. She was treated with ampicillin/sulbactam which led to the resol...

. Abstract: The benthic communities of fringing and mushroom-shaped shalIow-water reefs of the Abrolhos region (southern coast of Bahia) were surveyed. Line transects were used to estimate coral and alga1 percentage cover. Mussismilia braziliensis is the most conspicuous coral species in the majority of the communities surveyed, but turf algae make up the dominant cover in alI but one studied r...

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from mixed-species microbial mats, characterized and examined for metal tolerance and bioremediation potential. Contributing mats were natural consortia of microbes, dominated by cyanobacteria and containing several species of bacteria arranged in a laminar structure, stabilized within a gel matrix. Construc...