Pulsed field gel (PFG) electrophoresis can resolve DNA molecules as large as several million base pairs (mbp) in size (Schwartz and Cantor, 1984; Carle and Olson, 1984; Gardiner et al. 1986; Carle et al. 1986; Chu et al. 1986; Clark et al. 1988; Anand, 1986; Gardiner and Patterson, 1988; Orbach et al. 1988). This is in contrast to conventional electrophoresis where the practical upper limit is ...

The genomic DNA of 47 strains of TSST-1 toxin-producing Staphylococcus aureus were cleaved with SmaI restriction endonuclease and resolved in an agarose gel by pulsed-field gel electrophoresis (PFGE). An algorithm was designed to standardize the band weights or brightness (trace quantity) produced to a bounded region between 0 and 1 regardless of DNA fragment size while simultaneously reducing ...

We identified the serotypes and genomes patterns of 100 strains of Pseudomonas aeruginosa (P. aeruginosa) that had been isolated from patients who were admitted to the hospital of the Fukui Medical School between 1992 and 1995. A monoclonal diagnostic kit was used to identify the serotypes. Genome patterns were determined by pulsed field gel electrophoresis (PFGE). Serotypes A, B, C, D, E, F, G...

Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number of sites and were used for the determination of the genome sizes of wMelPop, ...

Viruses are the most abundant microorganisms in marine and freshwater environments and perhaps the most genetically diverse (Fuhrman and Suttle, 1993). Counting viruses in aquatic samples is now a routine matter, but assessing the diversity and dynamics within complex assemblages is still a challenge. DNA-based fingerprinting approaches, which rely on amplification of rRNA gene fragments by PCR...

Pulsed field gel electrophoresis allows separation and visualization of very large DNA molecules. This powerful technique is predominantly used in many aspects in molecular biology and medical diagnostics. Since the procedure for the preparation of the samples involves several steps, many different parameters can be changed and subsequently influence the resolution of the results. Therefore, in...

Cholera posed a significant threat causing outbreaks/epidemics with high morbidity and mortality in Odisha. This study envisages the characterisation of isolated pathogen from two cholera outbreaks reported 2018 2019 Bargarh Rayagada districts Odisha respectively. Vibrio cholerae O1 were following standard techniques. The different virulent drug resistant genes detected by multiplex PCR assays;...

Recently, we have reported on the isolation of a high molecular weight species of soluble elastin from lathyritic chick which was postulated to represent the primary precursor of elastin (proelastin). In order to establish the existence and precursor function of proelastin, second passage rabbit aortic smooth muscle cells, previously shown to be synthesizing insoluble elastin, were pulsed with ...