O polímero híbrido derivado de siloxano e quitosana foi obtido pela técnica sol-gel, tendo como precursor o tetraetilortosilicato (TEOS). O suporte híbrido obtido foi modificado quimicamente com epicloridrina e utilizado para imobilizar lipase de Burkholderia cepacia. O híbrido SiO2-quitosana deu origem a uma nova estrutura macromolecular na qual as partículas inorgânicas encontramse dispersas ...

Liquid chromatography/electrospray ionization/mass spectrometry (LC/ESI/MS) was used to determine intact phospholipid profiles for five reference pseudomonad strains harboring different (aerobic) toluene catabolic pathways: Pseudomonas putida mt-2, Pseudomonas putida F1, Burkholderia cepacia G4, Burkholderia pickettii PKO1, and Pseudomonas mendocina KR1. These five strains contained a predomina...

Application of exotic bacterial strains as biofertilizer has always posed a constraint in the success technology. Only limited number biofertilizers with applicability wide range soil conditions is available market. Pseudomonas cepacia BAM-12 (MTCC No. 7100) (now known Burkholderia cepacia), an isolate from mungbean rhizosphere agricultural field Rajasthan shown remarkable plant growth promotio...

Effects of various additives on the lipase from Burkholderia cepacia (BcL) immobilized on mixed-function-grafted mesoporous silica gel support by hydrophobic adsorption and covalent attachment were investigated. Catalytic properties of the immobilized biocatalysts were characterized in kinetic resolution of racemic 1-phenylethanol (rac-1a) and 1-(thiophen-2-yl)ethan-1-ol (rac-1b). Screening of ...

Human milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located positions sn-1,3. This study aimed production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis tripalmitin with acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized silica (prepared protic or apro...

The possibility of using the nutritionally versatile bacterium Pseudomonas cepacia to produce poly-beta-hydroxyalkanoic acid was evaluated. Chemostat culture showed that growth of P. cepacia became nitrogen limited when the molar carbon-to-nitrogen ratio of the medium fed into the fermentor was above 15. When grown under nitrogen limitation in batch culture with fructose as the sole source of c...