Abstract Phalaenopsis amabilis Blume var. grandiflora Bateman is economically important as cut and pot flower. Polyploidy considered a valuable tool in improvement evolution of ornamental plants. Protocorm-like bodies (PLBs) P. were cultured on Murashige Skoog medium containing 0.20 mg L-1 IBA together with 2.00 KIN 1.00 g activated charcoal grown for period five months. Fully-developed plantle...

An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l ...