نتایج جستجو برای: protein sequencing
تعداد نتایج: 1336591 فیلتر نتایج به سال:
Background and Objective: Ciliary neurotrophic factor (CNTF) was originally described as a trophic factor supporting the survival of ciliary ganglion neurons in vitro. CNTF can also promote the survival and differentiation of a variety of nerve cells including sensory, motor, autonomic neurons and oligodendrocytes. To enhance the neurotrophic effect of BMSCs, the CNTF-pSec-tag 2 /hygro A plasmi...
Introduction: Epithelial cell adhesion molecule (EpCAM) is a membrane glycoprotein that is overexpressed on the majority of tumor cells of epithelial origin and thereby can be used as a target of immunodetection and immunotherapy of cancer. So, it is important to produce this protein in its native form. Interestingly, during the last two decades, the yeast Pichia pastoris (P. pastoris) has beco...
Background: Analysis of many proteins produced during the transition into the stationary phase and under stress conditions (including starvation stress) demonstrated that a number of novel proteins were induced in common to each stress and could be the reason for cross-protection in bacterial cells. It is necessary to investigate the synthesis of these proteins during different stress condition...
Abstract We report comparisons between the complete genomic sequences of five historical Western Australian isolates subterranean clover mottle virus (SCMoV) from 1989–2000, and an infectious clone its 1989 isolate. Sanger Sequencing (SS) High Throughput (HTS), or both, were used to obtain these genomes. Four SCMoV sequenced by SS in 1999–2002, but re-sequenced again HTS 2020. The pairs obtaine...
Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in E. coli by a reliable, practical, simple and low cost method. Materials and Methods: In this study, the T...
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