Molecular docking analysis is routinely used in modern drug research to understand and predict the relationship between a molecule target protein from microbe. The entry replication of pathogens host cells can be prevented by drugs identified this way. coronavirus disease associated with SARS-CoV-2, COVID-19, has become today's most infectious lethal pandemic world. Burgeoning absence any parti...

Abstract Protease is a type of enzyme that hydrolyzes proteins into peptides and amino acids. The demand for protease green technology in some industries like detergent, food, leather increasing nowadays. Some soil bacteria, especially the Bacillus strains, are known to have ability produce protease. Compared other sources plant animal origin, microbial has more advantages as it can be produced...

Abstract Pathogenic autoantibodies are key effectors of inflammation, promoting complement cascade activation, immune cell response, and the consequent tissue damage in autoimmune disease transplant rejection. Proteases that cleave IgG antibodies present a novel therapeutic opportunity to treat diseases antibody mediated Using our IMPACT platform leveraging machine learning, we engineered prote...

Soil samples of Tehran jungle parks were screened for proteolytic Bacilli. Among eighteen protease producers one of the isolates obtained from Lavizan park, in north east of Tehran, was selected for further experimental studies. This isolate was identified as Bacillus sp. strain CR-179 based on partial sequencing of 16S rRNA. Various nutritional and environmental parameters affected protease pr...

Penicillium candidum (PCA 1/TT031) synthesizes different types of extracellular proteases. The objective of this study is to optimize polyethylene glycol (PEG)/citrate based on an aqueous two-phase system (ATPS) and Response Surface Methodology (RSM) to purify protease from Penicillium candidum (PCA 1/TT031). The effects of different PEG molecular weights (1500-10,000 g/mol), PEG concentration ...

Objective(s) Some properties of neuraminidase produced by Pseudomonas aeruginosa PAO1 growth in a defined medium (BHI) were examined and evaluated for its features. Materials and Methods The obtained supernatant enzyme of P. aeruginosa PAO1 cultures was used in a sensitive fluorometric assay by using 2'-(4-methylumbelliferyl) a-D-N acetylneuraminic acid as substrate. As hydrolyzing MUN with ...

Non-structural protein 3 (NS3) is a multifunctional enzyme possessing serine protease, NTPase, and RNA unwinding activities that are required for hepatitis C viral (HCV) replication. HCV non-structural protein 4A (NS4A) binds to the N-terminal NS3 protease domain to stimulate NS3 serine protease activity. In addition, the NS3 protease domain enhances the RNA binding, ATPase, and RNA unwinding a...

Proteases are being widely used in various industries like detergent, leather, food and pharmaceuticals.Protease was purified to homogeneity from the seeds of Artocarpus heterophyllus. The enzyme found be a tetramer having molecular mass 74 kDa. Gelatin zymography showed clear band proteolysis. isolated serine protease, as indicated by its inhibition with PMSF. stable at broad pH temperature ra...

Partially purified neutral protease was entrapped in calcium alginate beads and characterized using casein as a substrate. Temperature and pH maxima of the enzyme showed no changes before and after immobilization and remained stable at 50°C and 7.5 respectively. However substrate concentration for maximum enzyme activity of immobilized enzyme was shifted from 0.45% to 0.8%. Reaction time for im...

In the present study E. coli was used for the production of alkaline protease in eight different fermentation media. This bacterium was isolated from soil sample and screened for protease production. The enzyme production from E. coli was estimated using casein as substrate and its molecular weight was determined by SDS-PAGE. The enzyme activity was also studied at different parameters like pH,...