Double stranded cDNA molecules complementary to purified Rainbow trout protamine mRNA have been cloned in the bacterial plasmid pBR322. In order to circumvent the problems associated with a heterogeneous cDNA probe when identifying recombinants, a comparative hybridisation technique was used which can resolve between closely related cloned sequences. Using this technique, selected recombinants ...

We report the synthesis of a simple pyrene derivative and its application in protamine detection and monitoring of trypsin activity. This assay can be conducted in aqueous solution and features rapid response, visual detection, high sensitivity and selectivity. The limit of detection of protamine was 0.5 μg mL(-1). The IC50 value of a soybean trypsin inhibitor was estimated to be 0.51 U mL(-1).

Successful achievement of extracorporeal circulation requires that blood coagulability be temporarily interrupted during perfusion and afterward restored to normal. Heparin is accepted as the drug of choice in rendering the blood incoagulable, but there are two drugs, protamine and Polybrene (hexadimethrine bromide), available for effective in vivo neutralization of heparin, and controversy exi...