The standard amplified fragment-length polymorphism (AFLP) technique was modified to develop a convenient and reliable technique for rapid genetic characterization of plants. Modifications included (i) using one restriction enzyme, one adapter molecule and primer, (ii) incorporating formamide to generate more intense and uniform bands and (iii) using agarose gel electrophoresis. Sea oats (Uniol...

Efficient procedures are outlined for plant regeneration through direct shoot bud formation and indirect organogenesis through callus formation in Ornithogalum virens Lindl using bulb scale as explant. Murashige and Skoog’s (MS) medium containing 1 mg/L (5.4mM) NAA and 2 mg/L (4.4mM) BA was most effective in direct induction of shoot buds from explant. Callus cultures were raised from the bulb ...

In vitro asymbiotic seed germination of Dendrobium chrysotoxum varied with fruit harvesting time and culture medium used for germinating seeds. Seeds at different developmental stages were harvested and cultured on five asymbiotic orchid seed germination media i.e. defined and undefined media, namely 1⁄2 MS, KC, M, MS and PDA. Seeds harvested 220 days after pollination showed maximum germinatio...