The potency of naked DNA vaccines is limited by their inability to amplify and spread in vivo. VP22, a HSV-1 protein, has demonstrated the remarkable property of intercellular transport and may thus provide a unique approach for enhancing vaccine potency. Therefore, we created a novel fusion of VP22 with a model Ag, human papillomavirus type 16 E7, in a DNA vaccine that generated enhanced sprea...

The cancer potency of polygeenan was estimated from dose-response data of colorectal tumors among male and female rats exposed orally (Wakabayashi et al., 1978). The cancer potency estimate corresponds to the upper 95 percent confidence bound on the linear term of the multistage model fit to cancer dose-response data in animals. The potency derivation takes into account body size differences be...

Pyrovalerone cathinones are potent psychoactive substances that possess a pyrrolidine moiety. Pyrovalerone-type novel (NPS) continuously detected but their pharmacology and toxicology largely unknown. We assessed several pyrovalerone related cathinone derivatives at the human norepinephrine (NET), dopamine (DAT), serotonin (SERT) uptake transporters using HEK293 cells overexpressing each respec...

High-throughput in vitro screening experiments can be used to generate concentration-response data for large chemical libraries. It is often desirable to estimate the concentration needed to achieve a particular effect, or potency, for each chemical tested in an assay. Potency estimates can be used to directly compare chemical profiles and prioritize compounds for confirmation studies, or emplo...

BACKGROUND Therapeutic angiogenesis induced by the implantation of autologous bone marrow-derived cells has been used for the treatment of ischemic diseases. However, as the outcomes of cell implantation obviously vary among patients, it is essential to identify patients that would benefit the most from this treatment. METHODS AND RESULTS We collected clinical and laboratory data from 25 pati...

The cancer potency of 2-methylaziridine (propyleneimine) was estimated from the dose-response data of Weisburger et al. (1981). The cancer potency estimate corresponds to the upper 95 percent confidence bound on the linear term of the multistage model fit to cancer dose-response data in experimental animals. The potency derivation takes into account body size differences between humans and expe...

The University of Mississippi has a contract with the National Institute on Drug Abuse (NIDA) to carry out a variety of research activities dealing with cannabis, including the Potency Monitoring (PM) program, which provides analytical potency data on cannabis preparations confiscated in the United States. This report provides data on 46,211 samples seized and analyzed by gas chromatography-fla...

The potencies of six commercially manufactured heparins have been measured by the British Pharmacopoeial (BP) assay and activated partial thromboplastin time (APTT), protamine sulphate, and anti-Xa assays. The APTT/BP potency ratios were found to vary with the preparation but this was not dependent on the tissue source of heparin. For mucosal heparins, the anti-Xa/BP potency ratios were close t...

Epithelial sodium channel (ENaC) blockers have been proposed as a therapy to restore mucus clearance (MC) in cystic fibrosis (CF) airways. The therapeutic effects of the first generation ENaC blocker, amiloride, in CF patients, however, were minimal. Because the failure of amiloride reflected both its low potency and short duration of action on airway surfaces, we investigated whether the incre...

The cancer potencies of phenylhydrazine and phenylhydrazine hydrochloride were estimated from dose-response data from three data sets: lung tumors among male and female mice (combined) exposed orally to phenylhydrazine hydrochloride (Clayson et al., 1966) and blood vessel tumors among male and female mice exposed orally to phenylhydrazine hydrochloride (Toth and Shimizu, 1976). Studies of pheny...