Phosphoenolpyruvate (PEP) carboxylase was purified over 400-fold from Plasmodium berghei. The purified enzyme was stable in 0.4 m potassium phosphate buffer (pH 7.4) containing 0.5 m glucose, 1 mm ethylenediaminetetraacetic acid (EDTA), and 1 mm MgCl(2). It had a molecular weight of 280,000 determined by sucrose density gradient centrifugation in this buffer, but it aggregated and was unstable ...

In clinical laboratories, sometimes there is a need to recentrifuge the original tubes ("clot" tubes) in order to better clarify and clean the serum or plasma for further analysis. Also, the original tubes are recentrifuged to ensure there is an adequate volume of serum or plasma for multiple repeating or different tests, and/or to run additional tests that are ordered hours after the original ...

BACKGROUND The use of intravenous bicarbonate in diabetic ketoacidosis (DKA) may be considered for patients with a pH less than 6.9 according to the American Diabetes Association. The impact of this therapy on resolution of acidosis in patients with DKA is unclear. OBJECTIVE To determine whether the use of intravenous bicarbonate therapy was associated with improved outcomes in patients with ...

Severe hyperkalemia is a medical emergency that can cause lethal arrhythmias. Successful management requires monitoring of the electrocardiogram and serum potassium concentrations, the prompt institution of therapies that work both synergistically and sequentially, and timely repeat dosing as necessary. It is of concern then that, based on questions about effectiveness and safety, many physicia...

A 48-year-old Chinese presented with bilateral blurred vision, and was found to have accelerated hypertension and funduscopic manifestation of papilloedema. He had been diagnosed as having a left adrenal tumour 2 months previously, when he first reported anorexia and left upper quadrant pain. Computed tomography (Figure 1) and subsequent laparotomy excision revealed a large adrenocortical carci...

Optical techniques may potentially be used for noninvasive glucose sensing. We investigated the application of phase-sensitive optical low-coherence reflectometry (PS-OLCR) to the measurement of analyte concentrations. The dependence of the PS-OLCR signal on the concentration of various analytes, including aqueous solutions of glucose, calcium chloride, magnesium chloride, sodium chloride, pota...

In the Brigalow Belt bioregion of Australia, clearing brigalow (Acacia harpophylla) scrub vegetation for agriculture has altered nutrient cycling over millions hectares. order to quantify effect this and land use change on soil fertility, Catchment Study commenced in 1965. Initial burning resulted a temporary increase mineral nitrogen, total available phosphorus, exchangeable potassium sulfur s...

Dear Editor-in-Chief, We read with great interest the article entitled “A review of aluminium phosphide poisoning and a flowchart to treat it” written by Hashemi-Domeneh et al. (1) and recently published in the Archives of Industrial Hygiene and Toxicology. The authors mentioned performing a gastric lavage using a solution of potassium permanganate (1/10000/1 g per 10 L) and administering activ...