To improve the quality of frozen material during cryopreservation, scientists apply various effects on cells: mechanical, chemical or physical. In this work we use acoustic-mechanical cells before cryopreservation. As a result studies, optimal parameters impact piezoactuator were selected to defrosted reproductive male sturgeons. The object research was sperm Russian sturgeon. progressive motil...

One of the most significant indicators affecting male fertility is sperm nuclear and mitochondrial DNA fragmentation index (DFI). damage depends on biotic abiotic factors, leading to oxidative stress (O.S.). This research aimed investigate relationship between dysfunction spermatozoa their biological adequacy. The material was frozen-thawed samples from Ayrshire, Russian Black Pied Holstein, Re...

Introduction Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1780, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum condition...

Flow cytometry was utilized to measure the influx of calcium into fresh and frozen-thawed equine and bovine spermatozoa that had been treated with either dilauroyl-phosphatidylcholine ( PC-12) or the calcium ionophore A23187 (A23187) to initiate sperm capacitation. Sperm suspensions were stained with the fluorescent calcium indicator, Fluo-3, to measure intracellular calcium concentrations and ...

ABSTRACT This study aimed to evaluate the addition of different concentrations IGF-I and insulin egg yolk-based extender improve bovine semen cryopreservation. Two experiments were developed effects additives in two commercial extenders, Botubov® (Experiment 1) Triladyl® 2), both with same design. Three ejaculates from four bulls (n = 12) used. Each ejaculate was divided into seven equal fracti...

Many of the world's wild feline species are threatened by extinction. Gene banks are created with the aim of preserving their genetic materials. In case of unexpected death of the animals, epididymal sperm preservation can be used to avoid loss of genetic material. However, one requirement for the genetic preservation of endangered species is to preserve as much genetic material as possible. Th...

This study was done to determine the effects of processing techniques on the quality of semen from Dutch AI-bucks with the view on improving pregnancy rates after artificial insemination (AI) with liquid or frozen-thawed semen. Motility of spermatozoa was estimated under a microscope whereas the percentage live spermatozoa and the percentage live spermatozoa with intact acrosomes were determine...

The main objective of this study was to isolate high-quality total ribonucleic acid (RNA) from raw fresh semen and frozen-thawed boar semen, using a protocol comprising the conventional TRIzol assay and a membrane-based technique, the PureLink RNA mini kit. Bioanalyzer profile revealed that the sperm RNA size distributions comprised mainly intact RNA ranging from 1500 to 1800 bp, without any de...