We evaluated the Roche LightCycler Staphylococcus M(GRADE) kits to differentiate between Staphylococcus aureus and coagulase-negative staphylococci in blood cultures growing clusters of gram-positive cocci. Testing 100 bottles (36 containing S. aureus), the assay was 100% sensitive and 98.44% specific for S. aureus and 100% sensitive and specific for coagulase-negative staphylococci.

An enzyme-linked immunosorbent assay was developed for detection of immunoglobulin A (IgA) antibody to Bordetella pertussis (PsIgA) in nasopharyngeal secretions as an indicator of recent infection. Secretion specimens submitted for pertussis culture were examined for PsIgA by this technique. Of 348 specimens tested, B. pertussis was cultured from 57, and PsIgA was detected in 8 culture-positive...

Novel real-time PCR assays targeting the Bordetella pertussis insertion sequence IS481, the toxin promoter region and Bordetella parapertussis insertion sequence IS1001 were designed. PCR assays were capable of detecting ≤10 copies of target DNA per reaction, with an amplification efficiency of ≥90 %. From September 2003 to December 2009, per-nasal swabs and nasopharyngeal aspirates submitted f...

conclusions it was demonstrated that, considerable percentage of uti existed in the gastroenteritis diseases. early treatment of uti in patients would reduce uti complications. objectives the current study aimed to investigate uti in patients with diarrhea. patients and methods this case-control study was conducted on 200 participants, 100 were patients with acute gastroenteritis and the other ...

background: group b streptococcus (gbs) colonizes the gastrointestinal and genitourinary tract of 10-40% of pregnant women and it is a major cause of neonatal morbidity and mortality. objective: this study was to evaluate whether vaginal gbs culture results alter after digital vaginal examination or not. antibiotic resistance pattern of this specie has been also assessed. materials and methods:...

background & objectives: tuberculosis is one of the greatest health problems in iran. the distribution of the disease is not equal in all parts of the country. the aim of this study was to evaluate the frequency of positive results for mycobacterium tuberculosis in samples referred to an academic hospital in an 8 year period. materials and methods: the samples from different wards of qaem ...

BACKGROUND & OBJECTIVE Mycoplasma pneumoniae is known to be a major cause of lower respiratory tract infections in children. A specific diagnosis is important to institute the appropriate treatment. Information on diagnostic methods used for M. pneumoniae in Indian paediatric population is scarce. The study was thus conducted to compare polymerase chain reaction (PCR), culture and serology for ...

STUDY OBJECTIVES Identification of acid-fast bacilli (AFB) in the sputum smear at the completion of tuberculosis therapy is in some settings considered evidence of treatment failure. However, some patients with pulmonary tuberculosis (TB) will have positive smear results with negative sputum culture results at the end of therapy. The objectives of this study were to estimate the prevalence of p...