BACKGROUND Molting is an essential biological process throughout the life history of crustaceans, which is regulated by many neuropeptide hormones expressed in the eyestalk. To better understand the molting mechanism in Portunus trituberculatus, we used digital gene expression (DGE) to analyze single eyestalk samples during the molting cycle by high-throughput sequencing. RESULTS We obtained ...

The temperature of habitat water has a drastic influence on the behavioral, physiological and biochemical mechanisms of crustaceans. Hyperglycemia is a typical response of many aquatic animals to harmful physical and chemical environmental changes. In crustaceans increased circulating crustacean hyperglycemic hormone (CHH) and hyperglycemia are reported to occur following exposure to several en...

Green mussel shells (Perna viridis) and crab (Portunus pelagicus) have not been optimally utilized. It’s can be used as chitosan. Chitosan from green mussels has a low degree of deacetylation, so it needs to combined with in its manufacture. The aims this study were characterize determine the best combination raw materials for making chitosan shells. method completely randomized design triplica...

Persian Gulf supports diverse ecosystems and biota in need of remediation and protection and metal data from this region is needed. The levels of mercury in tissues of blue swimming crab, Portunus pelagicus and sediments from the Persian Gulf coasts, south Iran were investigated. Mercury analysis was performed by Atomic Absorption Spectrophotometer. The distribution pattern of mercury in the ti...

Genetic diversity and population differentiation of the blue swimming crab, Portunus pelagicus, in Thailand were analyzed by RAPD analysis. One hundred and twelve RAPD fragments were generated from 109 individuals of P. pelagicus using OPA02, OPA14, OPB10, UBC122, and UBC158 primers. The percentage of polymorphic bands in each geographic sample and that of each primer across overall samples wer...

The crab shell (Portunus pelagicus) has many benefits, one of the benefits is used in manufacture chitooligosaccharides. was chosen because it contains 20- 30% chitin inside. Chitin can be processed into chitosan and then hydrolyzed chitooligosaccharides using enzymes. This study aims to determine type enzyme that effective process hydrolyzing with various enzymes are α-amylase, lipase, cellula...

Demand from consumers for small quantities of live swimming crab Portunus trituberculatus is rising with the development e-commerce. However, it challenging to keep P. alive post-capture. In this study, a transport bag containing oxygen and seawater (24 ppt) was used investigate survival metabolic changes during transport. The results showed that more than 80% could survive at least 24 h in bag...

Trauma intensive care unit (TICU) patients requiring mechanical respiratory support frequently develop ventilator-associated pneumonia (VAP). Oral and oropharyngeal bacteria are believed to be responsible for many cases of VAP, but definitive evidence of this relationship is lacking. Earlier studies used conventional culture-based methods for identification of bacterial pathogens, but these met...