the accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. in this study, a gradient rp-hplc method is described for the separation of human growth hormone (hgh) variants as deamidated and oxidized forms. the methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1ml/min flow rate of a linear gradient of 0.1% v/...

spinal cord injury (sci) results in the damage of motor and sensory pathways. the molecular and cellular structure of the injury milieu has been shown to be vital for endogenous regeneration in most tissues. regeneration of injured central nervous system (cns) is limited by damaged cell environment. biomaterial scaffolds can reconstruct injured cns tissue following sci by promoting the migratio...

The demand for high-throughput and scalable cell expansion platforms that can accommodate diverse types remains a critical requirement across various biomedical fields. Fibronectin (Fn), an essential component of the extracellular matrix (ECM), has been used as conformal surface coating two-dimensional (2D) culture systems. However, soluble, globular Fn 2D coatings differs structurally from nat...

Additive manufacturing based on direct laser writing two-photon polymerization facilitates the fabrication of microstructures with full 3D design freedom. Here, this technique is exploited for engineering scaffolds accurately mimicking shape and size three types human cells. The cell models employed in study were chosen to include a range dimensions different identifiable features highlight ver...

Polycaprolactone (PCL) is one of the most recognized polymeric materials used for bone tissue engineering scaffold fabrication. This study aims to evaluate effects molecular weight (Mn) PCL on degradation kinematics, surface, microstructural, thermal, mechanical, and biological properties 3D printed scaffolds. Surface were investigated considering water-in-air contact angle nanoindentation test...

Introduction: The need for biocompatible and bioactive scaffolds to accelerate the regeneration and repair of fractured bones has been considered for bone tissue engineering applications during recent decades. The new methods were developed to produce scaffolds to improve the tissue quality, size of cavities, control the porosity and increase the scaffold compressive strength u...

In this work, the copolymer-based synthesized Cysteine-loaded nanocarriers prepared by a routine protocol, coprecipitation method. It is the first report to investigate the neuroprotective potential and biocompatibility of Cysteine derivatives loaded into poly(ethylene glycol)-block-poly(ε−caprolactone) methyl ether (PEG-b-PCL). The average size of the polymeric/empty NCs was 89 nm and for poly...

There are two main methods for preparing the PuraMatrix (PM) as a three-dimensional (3-D) scaffold for supporting the cells including surface plating and encapsulation. In the surface-plating procedure, the scaffold is created by adding an ionic agent such as medium to PM. After adjusting the PH by changing the medium, cells are seeded on top of the scaffold. In the encapsulation method, PM and...

Children use imitation flexibly to acquire the instrumental skills and conventions of their social groups. This study (N=69 parent and 3- to 6-year-old child dyads) examined the impact of instrumental versus conventional language on (a) children's imitative flexibility in the context of parent-child interaction and (b) how parents scaffold children's imitation. Children in dyads presented with ...