The present study was carried out to detect the presence of Alfalfa mosaic alfamovirus (AMV), cucumber mosaic cucumovirus (CMV), lettuce mosaic potyvirus (LMV), cucumber green mottle mosaic virus (CGMMV), tomato bushy stunt tombusvirus (TBSV), tobacco mosaic tobamovirus (TMV), tomato black ring nepovirus (TBRV) and tomato mosaic tobamovirus (ToMV) in seeds of pepper, tomato, cucumber and lettuc...

Powdery mildew (PM - Eryshpe necator) is one of the most important diseases threatening world viticulture. This study determined that gene expression levels Myc2, WRKY1, and DHN1a in Boğazkere (V. vinifera L. PM susceptible), Cabernet Sauvignon Kishmish Vatkana tolerant), Regent tolerant) cultivars after PM-Methyl jasmonate (MeJa) treatments by real-time polymerase chain reaction (RT-PCR). The ...

Abstract Background Coronavirus disease (COVID-19) presents in children usually with less severe manifestations than adults. Although fever and cough were reported as the most common symptoms, can have non-specific symptoms. We describe an infant aplastic anemia main manifestation. Case presentation a case of SARS-CoV-2 infection without any respiratory symptoms or signs while manifesting princ...

We performed serial nested reverse transcription polymerase chain reaction (RT-PCR) assays to monitor minimal residual disease (MRD) in 25 patients with t(8;21) acute myeloid leukemia (AML). We found transient disappearance of the AML1-ETO transcript in most of the patients in complete remission. Durable PCR-negativity was found in a subgroup of patients and was associated with a good prognosis.

We report a microfluidic platform for rapid screening and selection of monoclonal antibodies (mAbs) secreted by single cells. The microfluidic platform integrates single-cell handling and compartmentalization, fluorescence detection of antibodies secreted by cells, and cell recovery for reverse transcription polymerase chain reaction (RT-PCR) of antibody genes.

We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence and phylogenetic analysis confirmed the PCR product to have > or = 99% similarity to the WNV strain NY 2000-crow3356.

Abbreviations: attE: attacin E gene ELISA: Enzyme Linked Immunosorbent Assay gusA: β-glucuronidase gene GUS: glucuronidase nos: nopaline synthase gene nptII: neomycin phosphotransferase II gene NPTII: neomycin phosphotransferase II protein PCR: polymerase chain reaction PTGS: post-transcriptional gene silencing RNA: ribonucleic acid RT-PCR: reverse transcription PCR T-DNA: transfer DNA TGS: tra...

: The coronavirus disease 2019 (COVID-19) is a new infectious disease, firstly appeared in Wuhan city and has rapidly spread to 114 countries outside China, which receiving worldwide attention. As two important means of examination, computed tomography (CT) real-time reverse transcription polymerase chain reaction (RT-PCR) have always been controversial the clinical diagnosis COVID-19 pneumonia...

Rabies are one of the major zoonoses in Mali. The control this disease is based on use effective diagnostic techniques, management human cases after exposure and medical prophylaxis animals. Diagnosis usually made by detection viral antigen direct immunofluorescence (IF). Due to increasing variability genus Lyssavirus observed, it was found necessary a complementary technique, namely reverse tr...

In response to a recent outbreak in China, detection assays for a novel avian influenza A(H7N9) virus need to be implemented in a large number of public health laboratories. Here we present real-time reverse-transcription polymerase chain reaction (RT-PCR) assays for specific detection of this virus, along with clinical validation data and biologically-safe positive controls.