background: bordetella pertussis is the causative agent of pertussis. in brazil, laboratory diagnosis of pertussis is based on the culture. in 2010, was standardized the real-time pcr taqman® in routine diagnosis. objectives: the aim of this study was to evaluate the impact achieved with the introduction of rt-pcr for the routine diagnosis of pertussis and to compare with the results obtained f...

background: seborrheic keratoses (sk) are common, benign and often multiple skin tumors with disputed etiology. a follicular origin, late onset nevoid disturbance or local arrest in maturation of keratinocytes have been proposed. human papilloma virus (hpv) has been detected in a small number of cases, particularly those from the genital region. objectives: the aim of our study was to evaluate ...

by application of modern recombinant dna technology, especially the polymerase chain reaction (pcr)/dot-blot hybridization techniques, we have investigated the molecular basis of β-thalassemia from four different regions of iran: central, south-east, south and north. in this study, the dna samples were isolated from patients and for the identification of the mutations, the 6 oligonucleotide pro...

background: breast cancer is the most common malignancy in females worldwide. several etiological factors including environmental factors have been recognized for breast cancer. epstein barr virus as a viral etiological factor has been proposed. so far, several studies have investigated the relationship between development of breast cancer and epstein barr virus, but few have been done in iran....

background: toxoplasma gondii, as an important food-borne protozoan parasite, occurs widely in humans and animals including domestic poultry throughout the world. the goal of this survey was to determine prevalence rate of t. gondii in free-ranging chickens sold in tunis, tunisia. methods: in the present study, the prevalence of t. gondii in 40 free-range chickens (gallus domesticus) marketed i...

introduction: pneumocystis jiroveci is an opportunistic infectious fungus in immunosuppressed patients, particularly in ones with acquired immunodeficiency syndrome (aids). the use of immunosuppressive drugs especially corticosteroids predisposes the transplanted patients to a variety of infectious diseases including pneumocystis infection. in many developed countries, the incidence of pneumocy...

background: genital tuberculosis (gtb) is an important cause of female infertility, especially in developing countries. the positive results of polymerase chain reaction (pcr) in endometrial gtb in the absence of tubal damage raise the possibility of the detection of sub-clinical or latent disease, with doubtful benefits of treatment. objective: to evaluate the mycobacterium tuberculosis infect...

  Background and Objective: Sinusitis is one of the most common hygienic problems and a large part of the budget has been allocated to its diagnosis and treatment yearly. In this study, the presence of cytomegalovirus in sinuses of patients, undergone operation for treatment, with or without sinusitis, was studied. Materials and Methods: This study was an applied and case-co...

Polymerase Chain Reaction (PCR) merupakan uji diagnostik yang sangat sensitif. Enzim Taq salah satu komponen paling penting dalam pengujian (PCR). taq polymerase harganya mahal. Penggunaan pada PCR kurang efisien. Untuk mengetahui efisiensi penggunaan enzim dan volume terkecil berapa masih dapat digunakan metode PCR, maka dilakukanlah penelitian dengan membuat lebih kecil (5µl, 10 µl, 25 µl) da...