Since December 2019, COVID 19 pandemic has devastated communities across the world. As number of patients recovered from continue to rise, question acquired immunity versus chances re-infection becomes critical understand future spread infection. Here, we present a case patient previously COVID-19, develops new symptoms concerning for possible with positive reverse transcriptase-polymera...

PCR depends on generation of a deoxyoligonucleotide complementary to the known 3' terminus of the portion of DNA to be amplified. This is then annealed with the appropriate strand of heat-denatured DNA and used as a primer for synthesis by a DNA polymerase of a full-length complementary strand (Fig. 1). The two strands are then separated by heat denaturation and the process repeated. However, i...

Abbreviations: ALT: Alanine Transaminase; AST: Aspartate Transaminase; BMI: Body Mass Index; CMV: Cytomegalovirus; Cnis: Calcineurin Inhibitors; Csa: Cyclosporine; FK Or FK-506: Tacrolimus; GRWR: Graft Recipient Weight Ratio; HAI: Histological Activity Index; HCC: Hepatocellular Carcinoma; HCV: Hepatitis C Virus; HCV RNA: Hepatitis C Virus Ribonuclic Acid; HPB: Hepatopancreatobiliary;LDLT: Livi...

ObjectiveTo screen pregnant women at risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during delivery using reverse-transcription polymerase chain reaction (RT-PCR) test and serum immunoglobulin (Ig) testing.MethodBetween March 31 st August 2020, consecutive admitted for labor in a single hospital were screened SARS-CoV-2 with nasopharyngeal RT-PCR swab tests detec...

The single-cell RNA-seq allows exploring the transcriptome for one cell at a time. By doing so, cellular regulation is pictured. One limitation dropout events phenomenon, where gene observed low or moderate expression level in but not detected another. Dropouts obscure legitimate biological heterogeneity leading to description of small fraction meaningful relations. We used stochastic approach ...

Purpose: Prove that Computed Tomography (CT) is a more effective diagnostic tool for diagnosing Coronavirus Disease 2019 (COVID-19) in comparison with Reverse Transcription Polymerase Chain Reaction (RT-PCR) due to its higher sensitivity.
 Methods and materials: A total of 200 cases had records chest CT scans RT-PCR tests were collected from Palestinian governmental hospitals September Nov...

A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecti...

We passaged 52 serum samples from dengue patients on C6/36 cells for 7 days and checked the culture fluids by RT-PCR. Two serum samples, which were negative by direct RT-PCR, became positive. One sample was collected on fever day 1 and the other on fever day 2. Results indicate that combination of reverse transcriptase-polymerase chain reaction (RT-PCR) with passage of serum samples on C6/36 ce...

BACKGROUND This study was designed to compare the detection rates of conventional tumor markers with two molecular diagnostic approaches on blood samples from patients with esophageal squamous cell cancer. MATERIALS AND METHODS Preoperative blood samples were obtained from 44 esophageal cancer patients and were subjected to CEA-specific reverse transcriptase-polymerase chain reaction (RT-PCR)...