Recently, syngas has gained significant interest as renewable and sustainable feedstock, in particular for the biotechnological production of poly([R]-3-hydroxybutyrate) (PHB). PHB is a biodegradable, biocompatible polyester produced by some bacteria growing on the principal component of syngas, CO. However, working with syngas is challenging because of the CO toxicity and the explosion danger ...

Abstracts: The aim of the study was to evaluate the biocompatibility and bioactivity of two new prototype implants for bone tissue regeneration made from biodegradable fibrous materials. The first is a newly developed poly(l-lactide-co-glycolide), (PLGA), and the second is a blend of PLGA with synthetic poly([R,S]-3-hydroxybutyrate) (PLGA/PHB). The implant prototypes comprise PLGA or PLGA/PHB n...

Polymerization of â-butyrolactone (BBL) and â-valerolactone (BVL) using the zinc alkoxide initiator (BDI-1)ZnOiPr [(BDI-1) ) 2-((2,6-diisopropylphenyl)amido)-4-((2,6-diisopropylphenyl)imino)-2-pentene] proceeds very rapidly under mild conditions to produce poly(3-hydroxybutyrate) (PHB) and poly(3hydroxyvalerate) (PHV), respectively. For the monomer-to-initiator ratio 200:1, PHB number-average m...

Poly(3-hydroxybutyrate) (PHB) is a natural biopolyester accumulated in microbial cells as tiny amorphous granules. The nanomicro-particles have a variety of potential applications and behave differently in different environments. In this work, the in situ crystallization of native PHB granules was investigated under different environmental conditions. The isothermal crystallization kinetics of ...

The granules observed in the cytoplasm of cells of sporogenic and asporogenic strains of Clostridium botulinum type E were isolated at various developmental stages of growth and sporulation. Electron microscopy of thin sections showed that most of the granules were dispersed throughout the cytoplasm. Chemical analysis and electron microscopy showed that the granules were poly-beta-hydroxybutyra...

Intensive aquaculture releases large quantities of nutrients into aquatic bodies, which can lead to eutrophication. The objective of this study was the development of a biological recirculatory wastewater treatment system with a diazotrophic cyanobacterium, Aulosira fertilissima, and simultaneous production of valuable product in the form of poly-β-hydroxybutyrate (PHB). To investigate this pos...

The biodegradation of methane, a greenhouse gas, and the accumulation of poly-β-hydroxybutyrate (PHB) were studied using a methanotrophic consortium and an isolated strain thereof. The specific rates for methane consumption were 100 and [Formula: see text] for the isolate and the consortium, respectively. Also the effect of including 10% (vv(-1)) of silicone oil in a two-phase partitioning bior...

Industrial production of biodegradable polyesters such as polyhydroxyalkanoates is hampered by high production costs, among which the costs for substrates and for downstream processing represent the main obstacles. Inexpensive fermentable raw materials such as crude glycerol, an abundant by-product of the biodiesel industry, have emerged to be promising carbon sources for industrial fermentatio...

Methylobacterium extorquens AM1, a serine cycle facultative methylotroph, accumulates poly-beta-hydroxybutyrate (PHB) as a carbon and energy reserve material during growth on both multicarbon- and single-carbon substrates. Recently, the identification and mutation of the genes involved in the biosynthesis and degradation of PHB have been described for this bacterium, demonstrating that two of t...

Tissue engineering is based on the association of cultured cells with structural matrices and the incorporation of signaling molecules for inducing tissue regeneration. Despite its enormous potential, tissue engineering faces a major challenge concerning the maintenance of cell viability after the implantation of the constructs. The lack of a functional vasculature within the implant compromise...