A new rhenium tricarbonyl complex of a bis(quinoline)-derived ligand (2-azido-N,N-bis((quinolin-2-yl)methyl)ethanamine, L-N(3)), namely [Re(CO)(3)(L-N(3))]Br was synthesized and characterized in-depth, including by X-ray crystallography. [Re(CO)(3)(L-N(3))]Br exhibits a strong UV absorbance in the range 300-400 nm with a maximum at 322 nm, and upon photoexcitation, shows two distinct emission b...

1 Detection of antiviral-resistant hepatitis B virus (HBV) mutations is important for monitoring 2 the response to treatment and for effective treatment decision. We have developed an array 3 using Peptide Nucleic Acid (PNA) probes to detect point mutations in HBV associated with 4 antiviral resistance. PNA probes were designed to detect mutations associated with resistance 5 to lamivudine, ade...

Lymphocyte proliferation induced by the tumor promoter 12O-tetradecanoylphorbol-13-acetate (TPA) is inhibited by agents known to induce differentiation in murine erythroleukemia cells and other cell lines. In the present study, we determined the cellular targets for the action of TPA among murine thymocyte subpopulations, the phase of blastogenesis that is activated by the tumor promoter, and t...

Antisense oligonucleotide (ASO)-based drug development is gaining significant momentum following the recent FDA approval of Eteplirsen (an ASO based on phosphorodiamidate morpholino) and Spinraza (2'-O-methoxyethyl-phosphorothioate) in late 2016. Their attractiveness is mainly due to the backbone modifications which have improved the in vivo characteristics of oligonucleotide drugs. Another cla...

Aromatic peptide nucleic acid (APNA) monomers containing N-(2-aminobenzyl)-glycine, N-(2-aminobenzyl)-(R)- or -(S)-alanine, and N-(2-aminobenzyl)-beta-alanine moieties as part of their backbone were synthesized. These novel analogues were incorporated as a single "point mutation" in PNA hexamers, and their physicochemical properties were investigated by UV thermal denaturation and CD experiment...

Peptide nucleic acid (PNA) is a synthetic DNA mimic with valuable properties and a rapidly growing scope of applications. With the exception of recently introduced pseudocomplementary PNAs, binding of common PNA oligomers to target sites located inside linear double-stranded DNAs (dsDNAs) is essentially restricted to homopurine-homopyrimidine sequence motifs, which significantly hampers some of...

The combined time-dependent density functional theory effective fragment potential method (TDDFT/EFP1) is applied to a study of the solvent-induced shift of the lowest singlet π → π* charge-transfer excited state of p-nitroaniline (pNA) from the gas to the condensed phase in water. Molecular dynamics simulations of pNA with 150 EFP1 water molecules are used to model the condensed-phase and gene...

mouse liver tissue indicated the marked metabolic heterogeneity of the pentosenucleic acid (PNA) isolated from various cellular fractions. It was hoped that by extending such a study to tumor tis sue we could derive further information concern ing possible metabolic interrelationships between the PNA of various fractions and also between PNA and desoxypentosenucleic acid (DNA). Such information...

Measurements of the polarization dependence of hyper-Rayleigh scattering (HRS) by solutions of p-nitroaniline (PNA) in acetone-d6 were used to study molecular orientation correlations. The HRS observations were analyzed in terms of short range direct dipole-dipole correlations between the PNA molecules, a long range transverse polar mode for the PNA molecules, and a long range ion-induced longi...

BACKGROUND Recently, we introduced a novel peptide nucleic acid (PNA) multi-probe real time PCR method targeting the hsp65 gene (hsp65 PNA RT-PCR) to distinguish Mycobacterium abscessus groups. METHODS Here, we evaluated the usefulness of the hsp65 PNA RT-PCR for the direct identification of the M. abscessus group at the subspecies and genotype levels from sputa samples. The method was applie...