Light- and electron-microscopic observations of Ciona and Lytechinus spermatozoa show a thin terminal filament at the distal end. The terminal filament is 5-6 microns long and contains the two central microtubules and a variable number of A-tubule extensions of the peripheral doublet microtubules. The transition from the 9 + 2 region to the terminal filament is tapered more gradually in Lytechi...

KEY POINTS Omecamtiv mecarbil and blebbistatin perturb the regulatory state of the thick filament in heart muscle. Omecamtiv mecarbil increases contractility at low levels of activation by stabilizing the ON state of the thick filament. Omecamtiv mecarbil decreases contractility at high levels of activation by disrupting the acto-myosin ATPase cycle. Blebbistatin reduces contractility by stabil...

Extraction of SC-1 cells (African green monkey kidney) with the detergent Triton X-100 in combination with stereo high-voltage electron microscopy of whole mount preparations has been used as an approach to determine the mode of action of cytochalasin D on cells. The cytoskeleton of extracted BSC-1 cells consists of substrate-associated filament bundles (stress fibers) and a highly cross-linked...

We solve the free boundary problem for the dynamics of a cylindrical, axisymmetric viscoelastic filament stretching in a gravity-driven extensional flow for the Upper Convected Maxwell and Oldroyd-B constitutive models. Assuming the axial stress in the filament has a spatial dependence provides the simplest coupling of viscoelastic effects to the motion of the filament, and yields a closed syst...

Three methods of plastic embedding were assessed for their value in routine dermatopathology. The J B 4, Taab embedding, and Taab transmit techniques were compared for quality, convenience, and safety. The Taab transmit method was the most satisfactory method, being both rapid and simple. An additional advantage is that the resin can be removed and the immunoperoxidase technique carried out on ...

Dr. Pitman is Clinical Professor of Plastic Surgery, New York University School of Medicine, New York, NY. Dr. Aston is Surgeon Director and Chairman, Department of Plastiic Surgery, Manhattan Eye, Ear, and Throat Hospital and Professor of Plastic Surgery, New York University School of Medicine and Institute of Reconstructive Plastic Surgery, New York, NY. Dr. Feldman is Associate Clinical Prof...

Heat shock protein 90α plays a key role in myosin folding and thick filament assembly in muscle cells. To assess the structure and function of Hsp90α and its potential regulation by post-translational modification, we developed a combined knockdown and rescue assay in zebrafish embryos to systematically analyze the effects of various mutations on Hsp90α function in myosin thick filament organiz...