نتایج جستجو برای: plasmid construction

تعداد نتایج: 242846  

Journal: :Nucleic acids research 1991
H. Yoshimura Y. Ikeda M. Yoshimoto S. Tamaki K. Hanada T. Kusano T. Kohda H. Saito M. Oishi

L factor is a unique plasmid DNA which was originally discovered in a subclone (B822) of mouse L cells at a high copy number (more than 5,000 copies/cell). The presence of L factor caused no detectable abnormalities to the plasmid-bearing cells. We determined the total DNA sequence of the L factor I (and a part of L factor II) and compared it with that of polyoma DNA. Both DNA are common to the...

Journal: :Infection and immunity 1991
K S Fisher I H Maxwell J R Murphy J Collier L M Glode

We previously demonstrated that cells can be killed through transfection of an expression plasmid that encodes the diphtheria toxin A-chain fragment (DT-A). This report describes the construction of expression plasmids containing three mutant DT-A-coding sequences substituting glutamic acid 148 with aspartic acid, serine, or glutamine which are known to have 100- to 300-fold-reduced ADP-ribosyl...

Journal: :Applied and environmental microbiology 1997
M A Hefford Y Kobayashi S E Allard R J Forster R M Teather

As a preliminary step in the development of vector systems, we have isolated and begun to characterize small, cryptic plasmids from several strains of the rumen bacterium Butyrivibrio fibrisolvens. We present here the complete nucleotide sequence of Butyrivibrio plasmid pOM1, which was isolated from B. fibrisolvens Bu49. While it is very similar in size to the previously characterized Butyrivib...

Journal: :Journal of bacteriology 2000
N Firth S Apisiridej T Berg B A O'Rourke S Curnock K G Dyke R A Skurray

Based on structural and functional properties, three groups of large staphylococcal multiresistance plasmids have been recognized, viz., the pSK1 family, pSK41-like conjugative plasmids, and beta-lactamase-heavy-metal resistance plasmids. Here we describe an analysis of the replication functions of a representative of each of these plasmid groups. The replication initiation genes from the Staph...

Journal: :Molecular medicine reports 2012
Xianghui He Weidong Li Yifeng Wang Linan Hou Liwei Zhu

Interleukin-15 is a pleiotropic cytokine that has potential for cancer immunegene therapy. We previously reported the construction and characterization of IL-15 overexpression vectors pHi2-IL15-CMV-tat (L1) and pHi2-spIL15-CMV-tat (L3), as well as carcinoembryonic antigen promoter-amplified IL-15 expression plasmid vectors pHi2-IL15-CEA-tat (L2) and pHi2-spIL15-CEA-tat (L4). In the current stud...

Journal: :Journal of Venomous Animals and Toxins including Tropical Diseases 2007

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