It has been possible to duplicate in the hemophilic dog four of the major experiments which have suggested in humans an "anticephalin" hypothesis for the pathogenesis of hemophilia. The experiments in the dog have been considerably extended, as compared with the human experiments, by a variety of techniques. I. Asbestos was placed in contact with hemophilic dog plasma, and the clotting time bec...

objective: multiple myeloma (mm) is a plasma cell malignancy where plasma cells are increased in the bone marrow (bm) and usually do not enter peripheral blood, but produce harmful factors creating problems in these patients (e.g. malignant plasma cells over activate osteoclasts and inhibit osteoblasts with factors like rankl and dkk). these factors are a main cause of bone lesion in mm patient...

In this study, a novel method is described for the determination of tramadol in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacryl...

Micro dielectric barrier discharges (mDBDs) consist of micro-plasma devices (10–100 lm diameter) in which the electrodes are fully or partially covered by dielectrics, and often operate at atmospheric pressure driven with radio frequency (rf) waveforms. In certain applications, it may be desirable to extract electron current out of the mDBD plasma, which necessitates a third electrode. As a res...

A high performance liquid chromatography-inductively coupled plasma mass spectrometry method was developed for the fast and accurate analysis of arsenobetaine in fish samples extracted by accelerated solvent extraction. The combined extraction and analysis approach was validated using certified reference materials for arsenobetaine in fish and during a European intercomparison exercise with a b...

The competition between two substances for tubular excretion has been demonstrated repeatedly with orthodox clearance techniques. Smith, Goldring, and Chasis (1) found that raising the plasma concentration of diodrast depressed the excretion of phenol red. They concluded that both substances were excreted by the same mechanism. Similarly, it has been reported that intravenously administered dio...

A new method using high-performance liquid chromatography coupled with ultra violet detection (HPLC-UV) was developed and validated for the simultaneous quantification of atazanavir, dolutegravir, darunavir, efavirenz, etravirine lopinavir, raltegravir, rilpivirine and tipranavir in human plasma. For the first time we reported here the development and validation of an HPLC-UV assay to quantify ...

clomipramine is a tricyclic antidepressant. different methods for determination of clomipramine hydrochloride in plasma have been described. most of these procedures favor the use of acidic back-extraction in extraction procedure and hplc as the analytical technique. in this study, the clomipramine extraction procedure was modified and a direct injection to the column was performed to shorten t...

Solid-state extraction of plasma or serum offers significant advantages over traditional liquid-liquid methods in cortisol determinations by liquid chromatography (HPLC). Sample preparation is facilitated and HPLC column contamination is minimized. Extract yield from the solid-state columns is enhanced by sample pretreatment with methanol. Cortisol values by solid-state extraction/HPLC correlat...

I HE MICIIODETERMIXATION of fiee fatt acids (FFA) in plasma has received colisiderable attelition in recent years (1-8). Although a riumher of different procedures Ilave beemi reported, most of them consist of 2 essential steps: tire extraction of tire FFA from plasnla with orgamlic solvents, an(1 the titration of the extracted FFA in a 2-phase system with a standard aqueous base. Various extra...