An efficient and reproducible protocol for plantlet regeneration from nodal segments of Olive cv 'Frontio' has been developed. Media and explants browning due to exudation of phenolics from the explants were controlled by fortification of the medium with 100 mg/L ascorbic acid. Best establishment of olive explants was observed on half-strength MS salts fortified with 2.0 mg/L 6-benzylaminopurin...

For systematic and genome-wide analyses of rice gene functions, we took advantage of the full-length cDNA overexpresser (FOX) gene-hunting system and generated >12 000 independent FOX-rice lines from >25 000 rice calli treated with the rice-FOX Agrobacterium library. We found two FOX-rice lines generating green calli on a callus-inducing medium containing 2,4-D, on which wild-type rice calli be...

to produce embryogenic callus and somatic embryos, tendril explants were collected and cultured in ms medium supplemented with various concentrations of hormones. after one month most of explants produced callus. the highest percent of embryogenic callus obtained in ms medium supplemented with 4.5 µm bap and 1µm 2,4-d. production of somatic embryos was depended on genotype and medium. yaghouti ...

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (...

Light quality evaluations were conducted on yam (Dioscorea alata L. cultivar 'Oriental' ) plantlets to determine the potential usefulness of manipulation of the light environment in regulating growth. Eight-week old, in vitro grown yam plantlets were transferred to pots and treated in vivo with end-ofday (EOD) red (R) or far-red (FR) light. Also, nodal segments ofD. alata plantlets were culture...

Kultur in vitro adalah salah satu metode yang digunakan dalam perbanyakan tanaman pisang. Aklimatisasi merupakan masa adaptasi planlet dari kondisi ke ex vitro. Mikoriza diduga dapat meningkatkan kemampuan perakaran plantlet hasil kultur Penelitian ini bertujuan untuk mengetahui persentasehidup pisang pada tahap aklimatisasi serta menentukan dosis mikoriza terbaik pembesaran menggunakan Rancang...