The effect of different media including the growth regulators on somatic embryogenesis from Mexican lime was studied. The average embryo formation after 60 days from unfertilized ovules in different media was evaluated at 0 to 33.75%. The most effective hormone to stimulate embryogenesis was GA3 at 0.01 and 0.1 mg/l concentrations. Malt extract (ME) at 300 mg/l increased embryo formation, where...

The effect of different media including the growth regulators on somatic embryogenesis from Mexican lime was studied. The average embryo formation after 60 days from unfertilized ovules in different media was evaluated at 0 to 33.75%. The most effective hormone to stimulate embryogenesis was GA3 at 0.01 and 0.1 mg/l concentrations. Malt extract (ME) at 300 mg/l increased embryo formation, where...

In order to produce Tristeza virus-free plants from Satsuma mandarin (Citrus unshiu) cultivars (Ishikawa, Sugiyama and Myogawa), unfertilized ovules of plants suspected of CTV (Citrus Tristeza virus), from Mazandaran Mahdasht orchard were cultured on 4 media. Embryogenesis of nucellus tissue of ovules from Ishikawa and Sugiyama cultivars on MS medium were 31% and 25% and on MT medium from Ishik...