In the present investigation Jatropha and pea plant leafs was used, to germinated the plant by artificial condition through plant tissue culture method. We have taken three methods Method A, B & C to germinate callus, seed, bud and stem. 30 Sample were taken to analyze. In Method A 60% samples were contaminated (fungus and bacteria) and 20% was shown growth. In Method B 66% was contaminated by ...

Plants are an important source of natural flavourings for food. Plant-derived food flavours may be based on endogenous flavour compounds or be derived from flavour precursors following enzyme action when the tissue is disrupted or during a postharvest treatment. Such flavours are usually based either on a single compound or a small group of compounds with closely related chemical structure. How...

Article history: Received on: 25/12/2013 Revised on: 08/02/2014 Accepted on: 23/05/2014 Available online: 28/06/2014 The effect of tissue cultured and tissue culture derived Mentha species viz. Mentha piperita(PPR611), Mentha arvensis (SH) and Mentha spicata (SPR 8) on antioxidant potential using lipid peroxidation model was studied. The extracts prepared were analyzed for total phenols and fla...

Valerian (Valeriana officinalis L.) is an important medicinal plant that is widely exploited for its roots and rhizomes which contain valepotriates and valerenic acids (with putative pharmacological activities). Thus root proliferation of valerian is very important. The aim of this study was to establish a practical tissue culture method for rapid and large-scale induction of V. officinalis L. ...

Plant browning is a recalcitrant problem for in vitro culture and often leads to poor growth of explants and even failure of tissue culture. However, the molecular mechanisms underlying browning-induced physiological processes remain unclear. Medinilla is considered one of the most difficult genera for tissue culture owning to its severe browning. In the present study, intact aseptic plantlets ...

Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. Five T...

Medium composition for the callus induction, shoot multiplication in four Indian medicinal plants, Boerhaavia diffusa, Baliospermum axillare, Abutilon indicum and Nymphoides cristatum were standardized using Murashige and Skoog`s plant tissue culture medium as the basal medium. The protocol thus standardized was further evaluated replacing sucrose and vitamins with the fruit pulp of Borassus fl...