A direct staining technique was investigated for the detection of viable Salmonella in fresh and processed meats. The technique involved overnight enrichment in BPW, extraction of Salmonella cells onto a polycarbonate membrane, followed by detection of the pathogen using anti-Salmonella monoclonal antibody coupled with an antibody linked-fluorescent stain (Texas Red) and a viability stain (Syto...

Two carbonate stones (a limestone and a marble), commonly used for flooring, wall cladding countertops, were exposed to different staining agents. After each cycle (staining cleaning with non-ionic detergent solution), the surface degradation was assessed on scale ranging from unchanged severe change (based qualitative characterization presented standard UNE-EN 16301:2014), stereomicroscopy col...

The kidneys of a 3-month-old female cat were examined. The cat which had been raised under controlled conditions with no history of any poisoning showed progressive weight loss with increases in blood BUN and creatinine concentrations. At necropsy, both kidneys were firm in consistency with formation of focal scars. Histopathologically, widespread deposition of crystals was observed in the rena...

An unusual case of angiolymphoid hyperplasia with eosinophilia (ALHE) simulating port-wine stain in a 50-year-old woman is reported. The lesions of ALHE are typically papules or subcutaneous masses that range from light pink to red-brown in color. In addition to the usual histologic findings of ALHE, the biopsy in our patient showed some fibrin-like material and fibrous long-spacing collagen on...

BACKGROUND Currently available injectable fillers have demonstrated limited durability. This report proposes the in vitro culture of human adipose-derived stem cells (hASCs) on hyaluronic acid (HA) gel for in vivo growth of de novo adipose tissue. METHODS For in vitro studies, hASCs were isolated from human adipose tissue and were confirmed by multi-lineage differentiation and flow cytometry....

Fibrosis is the most common complication from chronic diseases, and yet no therapy capable of mitigating its effects available. Our goal to unveil specific signaling regulating fibrogenic process identify potential small molecule candidates that block differentiation fibro/adipogenic progenitors. We performed a large-scale drug screen using muscle-resident progenitors mouse model expressing EGF...

Melioidosis is a significant cause of morbidity and mortality in east Asia and northern Australia (8). Early diagnosis is vital if patients are to receive effective treatment. Definitive laboratory diagnosis is based on the isolation of Burkholderia pseudomallei, but culture is often slow or not available in these regions. Techniques such as direct immunofluorescence (DIF) (9) and urinary antig...