The effect of phytase on the performance, AMEn, and the ileal digestibility of N and amino acids was investigated in a 15-d trial using day-old male broilers with diets that were low in Ca (0.9% for control and 0.79% for phytase treatment) and available P (AP; 0.45% for control and 0.35% for phytase treatment). The assayed dietary phytase activity of crumble diet was 1,149 phytase unit (FTU)/kg...

A. niger XP isolated from Vietnam produces very low amount of acidic phytase with optimal pH at 2.5 and 5.5. The phytase production of this strain was successfully improved through gene cloning and expression. A 1.4 kb DNA fragment containing the coding region of the phyA gene was amplified by PCR and inserted into the expression vector pPICZαA with a signal peptide αfactor, under the control o...

In the current study, we report for the first time that grain components of barley, rice, wheat and maize can inhibit the activity of Aspergillus ficuum phytase. The phytase inhibition is dose dependent and varies significantly between cereal species, between cultivars of barley and cultivars of wheat and between Fusarium graminearum infected and non-infected wheat grains. The highest endpoint ...

A Less Polluting Pig There's good news in the fight against farm pollution, say researchers in the Department of Molecular Biology and Genetics at the University of Guelph in Ontario, Canada. In August, John Phillips, Cecil Forsberg, and Serguei Golovan announced a new kind of pig that makes better use of phosphorus, a nutrient that in high concentrations can deplete oxygen levels in waters dow...

Strong resistance to proteolytic attack is important for feed enzymes. Here, we selected three predicted pepsin cleavage sites, L99, L162, and E230 (numbering from the initiator M of premature proteins), in pepsin-sensitive HAP phytases YkAPPA from Yersinia kristensenii and YeAPPA from Y. enterocolitica, which corresponded to L99, V162, and D230 in pepsin-resistant YrAPPA from Y. rohdei. We con...

BACKGROUND Phytic acid and phytates can interact with biomolecules, such as proteins and carbohydrates, and are anti-nutritional factors found in food and feed. Therefore, it is necessary to remove these compounds in food and feed processing. Phytase can hydrolyze phytic acid and phytates to release a series of lower phosphate esters of myoinositol and orthophosphate. Thus, the purification and...

background: old laying hens are more prone to dietary calcium (ca) and phosphorus (p) deficiencies as  they absorb ca and p less efficiently than younger hens. objectives: in a 2×2 factorial design, the influence of diets with two levels of available phosphorus (ap) and phytase enzyme on the laying performance, egg quality and tibia bone characteristics of laying hens at their late 2nd producti...

BACKGROUND Phytase is used as an animal feed additive that degrades phytic acid and reduces feeding costs and pollution caused by fecal excretion of phosphorus. Some phytases have been expressed in Pichia pastoris, among which the phytase from Citrobacter amalonaticus CGMCC 1696 had high specific activity (3548 U/mg). Improvement of the phytase expression level will contribute to facilitate its...

This study investigated the metabolizable energy (ME) intake, net energy of production (NEp), heat production (HP), efficiencies of ME use for energy, lipid and protein retention as well as the performance of broiler chickens fed diets based on cassava chips or pellets with or without supplementation with an enzyme product containing xylanase, amylase, protease and phytase. The two products, ca...