Multiple stains have been historically utilized in electron microscopy to provide proper contrast and superior image quality enabling the discovery of ultrastructures. However, use these microbiological viability assessment has limited. Phosphotungstic acid (PTA) staining is a common negative stain used scanning (SEM). Here, we investigate feasibility new SEM-PTA assay, aiming determine both vi...

HE ESTABLISHMENT AND ACCEPTANCE of an analytic procedure as a method to be relied upon with complete confidence demands: (1) 8pecific’tty of the method, i.e. minimal interference by other compounds; (2) quantitative recovery of the specific substance when it is added to the medium in which it is usually determined; and (3) reproducibility of results within reasonable limits on replicate samples...

The objectives of this research are twofold: (a) to review current methods for rapid analysis dissolved protein in water and wastewater (b) introduce a new spectrophotometrical method determination proteins the 10–2500 mg/L range using carbonate-tartrate reagent, phosphomolybdic-phosphotungstic copper sulfate.

We describe the quantitative densitometric determination of phosphatidylcholine (PC) and sphingomyelin (SP) in human serum after precipitation with phosphotungstic acid/MgCl2 and use of thin-layer chromatography. After development, chromatographic plates were charred with methanolic sulfuric acid and MnCl2 and scanned by direct reflectance densitometry in an automated densitometric system inter...

Wettability is an important factor affecting the performance of catalytic oxidative desulfurization. In order to develop efficient catalyst for extractive coupled desulfurization (ECODS) fuel oil by H2O2 and acetonitrile, a novel family imidazole-based polyoxometalate dicationic ionic liquids (POM-DILs) [Cn(MIM)2]PW12O40 (n = 2, 4, 6) was synthesized modifying phosphotungstic acid (H3PW12O40) w...